TISSUE DISTRIBUTION OF THE HUMAN GLUT3 GLUCOSE TRANSPORTER
TISSUE DISTRIBUTION OF THE HUMAN GLUT3 GLUCOSE TRANSPORTER
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DOI：
10.1210/en.132.6.2538
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发表时间：
1993-06-01
期刊：
影响因子：
4.8
通讯作者：
MORGELLO, S
中科院分区：
文献类型：
--
作者：
HABER, RS;WEINSTEIN, SP;MORGELLO, S
使用针对人GLUT 3的C-末端肽序列的兔抗血清检查人组织中GLUT 3葡萄糖转运蛋白的组织分布。这种抗血清被证明可以识别GLUT 3 cDNA转染的中国仓鼠卵巢细胞中的人GLUT 3蛋白，并通过[H-3]细胞松弛素-B的葡萄糖可降解光标记评估，在脑和睾丸膜中免疫沉淀真实的葡萄糖转运蛋白。GLUT 3蛋白，迁移的表观摩尔重量约48千道尔顿，强烈表达在脑和睾丸膜以及精子。在红细胞、脂肪细胞、心脏、骨骼肌、肝、肾、脾、甲状腺和前列腺的膜中未检出。胎盘中可能存在极低水平。在脑中，GLUT 3蛋白在灰质区域中强表达，而在白色物质中仅弱表达，这表明它可能在向高代谢活性区域，即与突触传递相关的区域提供葡萄糖方面很重要。周围（股）神经未见异常。在4 ℃下保存的尸检脑组织中，它似乎稳定长达47 h。因此，与先前的报告相比，人GLUT 3蛋白的组织分布似乎高度受限（脑和睾丸/精子）。其功能可能是在高度依赖葡萄糖作为燃料源的细胞中提供高亲和力葡萄糖转运系统。
The tissue distribution of the GLUT3 glucose transporter protein was examined in human tissues using a rabbit antiserum directed against the C-terminal peptide sequence of human GLUT3. This anti-serum was shown to recognize the human GLUT3 protein in Chinese hamster ovary cells transfected with GLUT3 cDNA and to immunoprecipitate an authentic glucose transport protein in brain and testis membranes, as assessed by glucose-inhibitable photolabeling with [H-3] cytochalasin-B. The GLUT3 protein, migrating with an apparent mol wt of approximately 48 kilodaltons, was strongly expressed in brain and testis membranes as well as in spermatozoa. It was not detectable in membranes from erythrocytes, adipocytes, heart, skeletal muscle, liver, kidney, spleen, thyroid, and prostate. Very low levels may be present in placenta. In brain, GLUT3 protein was strongly expressed in grey matter regions and was only weakly expressed in white matter, suggesting that it may be important in providing glucose to regions of high metabolic activity, i.e. to areas associated with synaptic transmission. None was found in peripheral (femoral) nerve. It appeared to be stable for up to 47 h in autopsy brain tissue kept at 4 C.The tissue distribution of human GLUT3 protein thus appears to be highly restricted (brain and testis/spermatozoa), in contrast with a previous report. Its function may be to provide a high affinity glucose transport system in cells that are highly dependent on glucose as a fuel source.