Dietary alanyl-glutamine and vitamin E supplements could considerably promote the expression of GPx and PPARα genes, antioxidation, feed utilization, growth, and improve composition of juvenile cobia
Dietary alanyl-glutamine and vitamin E supplements could considerably promote the expression of GPx and PPARα genes, antioxidation, feed utilization, growth, and improve composition of juvenile cobia
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日粮中添加丙氨酰谷氨酰胺和维生素E可显着促进军曹鱼幼鱼GPx和PPARα基因的表达、抗氧化、饲料利用率、生长和改善成分
DOI：
10.1016/j.aquaculture.2016.12.015
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发表时间：
2017-03-01
期刊：
影响因子：
4.5
通讯作者：
Xu, Youqing
中科院分区：
文献类型：
--
作者：
Ding, Zhaokun;Li, Weifeng;Xu, Youqing
本试验旨在研究饲粮中添加不同丙氨酰-谷氨酰胺二肽（AGD）和/或维生素E （VE）对军械鱼幼鱼谷胱甘肽过氧化物酶（GPx）和过氧化物酶体增殖物激活受体α (PPAR α)基因表达、抗氧化、饲料利用、生长和组成的影响。6组幼鱼，每组3次，采用6种饲料饲喂12周：对照组饲料（DO）只含基本成分；日粮1 （D1）单次添加AGD 10 g kg（-1）干饲料；日粮2 （D2）单次添加100 IU kg（-1）干饲料；日粮3 （D3）添加AGD 10 g kg（-1）干饲料和VE 100 IU kg（-1）干饲料；日粮4 （D4）添加AGD 5 g kg（-1）干饲料和VE 50 IU kg（-1）干饲料；日粮5 （D5）添加AGD 2.5 g kg（-1）干饲料和VE 25 IU kg（-1）干饲料。在第0周和第12周随机抽取幼鱼进行分析。结果表明：饲料中添加AGD和VE可显著提高饲料利用率，促进幼鱼生长，改善其组成；AGD和VE联合添加可显著提高幼鱼肝脏和肌肉中谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）、谷胱甘肽s -转移酶（GST）、总抗氧化能力（T-AOC）、PPARa和GPx基因表达量（P < 0.05），显著降低丙二醛（MDA）含量（P < 0.05）；单独添加AGD对肝脏GPx基因表达的影响显著大于单独添加VE (P < 0.05)；而单独添加VE对肝脏和肌肉中PPARa基因表达的影响显著大于单独添加AGD （P < 0.05）。综上所述，AGD和VE联合添加可显著促进斗鱼幼鱼GPx和PPARa基因的表达、抗氧化、饲料利用和生长，改善鱼体组成；每kg干饲料添加5 g AGD和50 IU VE， D4饲喂效果最佳。相关性声明：由于结果相互矛盾，有必要评估在受控条件下对虱子沉降的光效应。我们分析了虱子沉降，同时考虑了先前存在的鳍侵蚀和鱼的大小。研究结果证实，光不是虱子侵扰的必要条件。鱼鳍侵蚀影响配对鱼鳍上的虱子数量，这表明将鱼鳍侵蚀作为潜在的生物物理参数考虑的重要性(C) 2016出版由Elsevier B.V.
This experiment was conducted to study the effect of various dietary alanyl-glutamine dipeptide (AGD) and/or vitamin E (VE) supplement(s) on the expression of glutathione peroxidase (GPx) and peroxisome proliferator-activated receptor alpha (PPAR alpha) genes, antioxidation, feed utilization, growth, and composition of juvenile cobia (Rachycentron canadum). Six groups of the juveniles in triplicate were fed for 12 weeks using 6 diets: Control diet (DO) contained only basic ingredients; Diet 1 (D1) contained single added AGD 10 g kg(-1) dried feed; Diet 2 (D2) contained single added VE 100 IU kg(-1) dried feed; Diet 3 (D3) contained added AGD 10 g kg(-1) dried feed and VE 100 IU kg(-1) dried feed; Diet 4 (D4) contained added AGD 5 g kg(-1) dried feed and VE 50 IU kg(-1) dried feed; Diet 5 (D5) contained added AGD 2.5 g kg(-1) dried feed and VE 25 IU kg(-1) dried feed. The juveniles were sampled randomly for analysis in week 0 and week 12. The main results were as follows: the diets with combined AGD and VE supplements could considerably promote feed utilization, growth, and improve composition of the juveniles; the combined AGD and VE supplements could significantly promote (P < 0.05) glutathione peroxidase (GPx), catalase (CAT), glutathione S-transferase (GST), total antioxidant capacity (T-AOC), the expression of PPARa and GPx genes, and significantly decrease (P < 0.05) malondialdehyde (MDA) in the liver and muscle of the juveniles; the effect of AGD supplement alone on the expression of GPx gene was significantly greater (P < 0.05) than that of VE supplement alone in the liver; whereas the effect of VE supplement alone on the expression of PPARa gene was significantly greater (P < 0.05) than that of AGD supplement alone in the liver and muscle. We concluded that the combined AGD and VE supplements could cooperate and considerably promote the expression of GPx and PPARa) genes, antioxidation, feed utilization, growth, and improve composition of juvenile cobia; the optimum was D4, with 5 g of AGD and 50 IU of VE added per kg of dried feed.Statement of relevance: There is a need to assess light effect on lice settlement in controlled conditions due to conflicting results. We analyzed lice settlement while taking into account preexisting fin erosion and fish size. Findings confirm that light is not requirement for lice infestation. Fin erosion influenced the number of lice on paired fins suggesting the importance of considering fin erosion as a potential biophysical parameter (C) 2016 Published by Elsevier B.V.