Identification of additional histaminergic neurons in Aplysia: Improvement of single cell isolation techniques for in tandem physiological and chemical studies
Identification of additional histaminergic neurons in Aplysia: Improvement of single cell isolation techniques for in tandem physiological and chemical studies
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海兔中其他组胺能神经元的鉴定：用于串联生理和化学研究的单细胞分离技术的改进
DOI：
10.1016/0306-4522(80)90152-9
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
R. Mccaman
中科院分区：
文献类型：
--
作者：
J. Ono;R. Mccaman
识别神经元的海洋软体动物,海兔californica,被用来改善forin tandemphysiological隔离技术和化学研究相同的神经元。先前的一项研究表明，经过细胞内记录和染色的组胺能神经元RC-2和LC-2对用于分离神经元以进行后续化学分析的冷冻替代程序特别敏感。本报告描述了冷冻替代程序的改进，该程序允许在生理操作的C-2神经元中极好地恢复组胺。他们的联合生理和化学程序开发鉴定神经元被用来表征额外的组胺含有神经元，不能通过单独的视觉检查识别。这些新的组胺能神经元，被命名为RC-3和LC-3，位于大脑神经节的腹侧表面，与其他邻近的含有组胺的神经元区分开来，因为它们与之前描述的RC-2和LC-2的一些相同的跟随神经元有单突触连接。本研究的结果进一步支持了组胺作为一种多作用神经递质的作用，并证明了在细胞内记录后测量神经元体内各种神经递质内源性水平的可行性。
Identifiable neurons in the marine mollusc,Aplysia californica, were used to improve isolation techniques forin tandemphysiological and chemical studies of the same neuron. A previous study demonstrated that the histaminergic neurons, RC-2 and LC-2, which had been subjected to intracellular recording and staining, were particularly sensitive to a freeze-substitution procedure used in isolating neurons for subsequent chemical assay. The present report describes an improvement of the freeze-substitution procedure which permits excellent recovery of histamine in physiologically manipulated C-2 neurons. Thein tandemphysiological and chemical procedures developed for identified neurons were used to characterize additional histamine-containing neurons which cannot be identified by visual inspection alone. These new histaminergic neurons, designated RC-3 and LC-3, are located on the ventral surface of the cerebral ganglion and are distinguishable from other adjacent histamine-containing neurons by their monosynaptic connections with some of the same follower neurons previously described for RC-2 and LC-2.The results of this study further support the role of histamine as a multiaction neurotransmitter and demonstrate the feasibility of measuring endogenous levels of various neurotransmitters in neuronal somata subsequent to intracellular recording.