Hydroxyproline-O-glycosylated peptide tags enhance recombinant protein yields in tobacco transient expression
Hydroxyproline-O-glycosylated peptide tags enhance recombinant protein yields in tobacco transient expression
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DOI：
10.1016/j.procbio.2013.12.010
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
Xu, Jianfeng
中科院分区：
文献类型：
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作者：
Dolan, Maureen C.;Wu, Di;Xu, Jianfeng
植物瞬时表达为重组蛋白提供了快速生产平台，但与低蛋白产量相关。为了测试附着在靶蛋白上的植物特异性羟脯氨酸(Hyp)- o糖基化肽标签是否能提高在benthamiana中瞬时表达的重组蛋白的总体产量，增强的绿色荧光蛋白（EGFP）被表达为与5或32个丝氨酸-脯氨酸基序的串联重复序列融合，命名为（SP）(5)或（SP）（32），已知其在植物中指导广泛的羟脯氨酸- o糖基化。含有（SP）(n)基序的EGFP产量增加的顺序如下：EGFP < EGFP-(SP)(5)
Plant transient expression provides a rapid production platform for recombinant proteins but is linked with low protein yields. To test if plant-specific hydroxyproline (Hyp)-O-glycosylated peptide tags attached to a target protein can improve overall yields of recombinant protein transiently expressed in Nicotiana benthamiana, enhanced green fluorescence protein (EGFP) was expressed as a fusion with 5 or 32 tandem repeats of a serine-proline motif, designated (SP)(5) or (SP)(32), which is known to direct extensive Hyp-O-glycosylation in plants. EGFP containing the (SP)(n) motif showed enhanced yields in the order as follows: EGFP < EGFP-(SP)(5)