Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms
Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms
复制标题
微生物生产化学品的底盘工程：从天然微生物到合成生物
DOI：
10.1016/j.copbio.2020.06.013
复制
发表时间：
2020
影响因子：
7.7
通讯作者：
Jian Zha
中科院分区：
文献类型：
--
作者：
Jingyi Liu;Xia Wu;Mingdong Yao;Wenhai Xiao;Jian Zha
亮点描述了修饰底盘微生物的常用策略。描述了合成基因组、人工核糖体、非天然碱基对和扩展密码子。具有合成机器的底盘展示了构建高效微工厂的强大能力。讨论了天然底盘工程的前体工程、耐受工程和辅因子工程。底盘为异源途径基因的表达提供了环境，这通常需要广泛的工程才能实现完整的功能。传统上，底盘工程依靠基因缺失/过度表达来调节前体/辅因子供应和产物运输，已产生了数千种高性能菌株。随着合成生物学的发展，底盘修饰已扩展到人工细胞机器的合成，为生物产品的生物合成创造合成细胞。在这篇综述中，我们将讨论底盘工程技术（称为第一代和第二代技术）的发展，及其在生产增值化学品的底盘中的应用，重点是合成底盘及其应用和潜力。底盘工程技术的发展将促进用于生产目标生物制品的定制底盘的合理设计和建造。
HighlightsThe commonly used strategies to modify chassis microbes are described.Synthetic genomes, artificial ribosomes, unnatural base pairs, and expanded codons are described.Chassis with synthetic machineries demonstrates powerful capability of constructing efficient microfactories.Precursor engineering, tolerance engineering, and cofactor engineering for engineering of natural chassis are discussed.Chassis provides a setting for the expression of heterologous pathway genes, which often requires extensive engineering to achieve complete functions. Traditionally, chassis engineering relies on gene deletion/overexpression for the regulation of precursor/cofactor supply and product transportation, which has generated thousands of high-performance strains. With the development of synthetic biology, chassis modifications have expanded to the synthesis of artificial cellular machineries, creating synthetic cells for the biosynthesis of bioproducts. In this review, we will discuss the development of chassis engineering technologies, termed the first-generation and second-generation technologies, and their applications in the creation of chassis for the production of valued-added chemicals, with an emphasis on synthetic chassis and their applications and potential. The development of chassis engineering technologies will advance rational design and construction of customized chassis for the manufacturing of target bioproducts.