Human Adipose Tissue Macrophages: M1 and M2 Cell Surface Markers in Subcutaneous and Omental Depots and after Weight Loss
Human Adipose Tissue Macrophages: M1 and M2 Cell Surface Markers in Subcutaneous and Omental Depots and after Weight Loss
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DOI：
10.1210/jc.2009-0925
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发表时间：
2009-11-01
影响因子：
5.8
通讯作者：
Clement, Karine
中科院分区：
文献类型：
--
作者：
Aron-Wisnewsky, Judith;Tordjman, Joan;Clement, Karine
背景：巨噬细胞聚集在脂肪组织中，可能参与肥胖的代谢并发症。巨噬细胞数量随脂肪组织部位和体重减轻而不同，但是否伴随表型变化尚不清楚。目的：研究肥胖患者脂肪组织巨噬细胞的激活状态。设计/地点：单中心前瞻性研究。参与者/干预：16例绝经前肥胖妇女(年龄41.1±8.6岁；体重指数43.8±3.4 kg/m(2))，在胃手术期间取皮下和大网膜脂肪组织的活检标本。3个月后，肥胖者和10名非肥胖者(年龄43.3±3.5岁；体重指数22.5±0.75 kg/m(2))接受皮下脂肪组织活检。用免疫化学方法检测CD40、CD206和CD163表面标记的巨噬细胞数量。主要结果：随着肥胖的发生，CD40(+)的巨噬细胞数量显著增加，肥胖女性的大网膜脂肪组织中CD40(+)巨噬细胞的数量显著高于sc脂肪组织。CD163(+)和CD206(+)巨噬细胞计数与肥胖和脂肪垫解剖位置无关。胃手术后3个月，肥胖者皮肤脂肪组织中CD40(+)/CD206(+)巨噬细胞的比率比手术前低2倍(P<；0.001)，这是由于同时伴有CD40(+)和CD206(+)巨噬细胞数量的增加。结论：肥胖者脂肪组织巨噬细胞的激活状态在胃旁路手术后3个月向M1状态倾斜，并转向较少的促炎状态。(临床内分泌代谢酶94：4619-4623,2009)
Context: Macrophages accumulate in adipose tissue and possibly participate in metabolic complications in obesity. Macrophage number varies with adipose tissue site and weight loss, but whether this is accompanied by phenotypic changes is unknown.Objective: The objective of the study was to characterize the activation state of adipose tissue macrophages in human obesity.Design/Setting: We performed a single-center prospective study.Participants/Interventions: Paired biopsies of sc and omental adipose tissue were obtained during gastric surgery in 16 premenopausal obese women (aged 41.1 +/- 8.6 yr; body mass index 43.8 +/- 3.4 kg/m(2)). Subcutaneous adipose tissue biopsies were obtained 3 months later in obese subjects and in 10 nonobese women(aged 43.3 +/- 3.5 yr; body mass index 22.5 +/- 0.75 kg/m(2)). The number of macrophages stained with CD40, CD206, and CD163 surface markers was determined by immunochemistry.Main Outcomes: The number of CD40(+) macrophages significantly increased with obesity and in omental vs. sc adipose tissue in obese women. No significant changes in CD163(+) and CD206(+) macrophage counts was found with obesity and fat pad anatomical location. Three months after gastric surgery, the ratio of CD40(+) to CD206(+) macrophages was 2-fold lower than before surgery in the sc adipose tissue of obese subjects (P < 0.001) due to a concomitant decrease of CD40(+) and increase of CD206(+) macrophages counts.Conclusion: We suggest that the activation state of adipose tissue macrophages is weighted toward M1 over M2 status in obese subjects and switch to a less proinflammatory profile 3 months after gastric bypass. (J Clin Endocrinol Metab 94: 4619-4623, 2009)