Medullasin enhances human natural killer cell activity.
Medullasin enhances human natural killer cell activity.
复制标题
髓质素增强人类自然杀伤细胞活性。
DOI：
10.1172/jci110561
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
K. Oshimi
中科院分区：
文献类型：
--
作者：
Y. Aoki;M. Sumiya;K. Oshimi
髓蛋白酶是一种在骨髓细胞中发现的新的丝氨酸蛋白酶，能显著增强人自然杀伤细胞的活性。尽管在用瑞拉新处理后立即测量的自然杀伤细胞活性几乎保持在与对照相同的水平上，但在37 ℃下孵育数小时显著增加了用瑞拉新处理的淋巴细胞的自然杀伤细胞活性。用生理浓度的蛋白酶（5-20微克/ml）处理引起天然细胞毒性的增强。抑制剂如苯甲基磺酰氟和弹性蛋白质的抑制剂阻止了天然细胞毒性的激活。消耗携带IgG的Fc受体的淋巴细胞，取消了阿托伐他汀对自然杀伤细胞活性的增强。干扰素活性未检测到在淋巴细胞培养物的上清液中刺激与克拉霉素。进一步增强了干扰素刺激的淋巴细胞的自然杀伤细胞活性。在未受刺激或受刺激（伴刀豆球蛋白A或植物血凝素）的人淋巴细胞中均未检测到髓蛋白酶活性。该蛋白酶易于从人成熟粒细胞释放到培养基中。从这些结果可以认为，人自然杀伤细胞活性水平受成熟粒细胞释放的白细胞介素调节。
Medullasin, a new serine protease found in bone marrow cells, increased markedly human natural killer cell activity. Whereas the natural killer cell activity measured immediately after the treatment with medullasin remained almost on the same level as the control, an incubation at 37 degrees C for several hours increased markedly the natural killer cell activity of the lymphocytes treated with medullasin. Enhancement of the natural cytotoxicity was caused by the treatment with physiologic concentrations of the protease (5-20 micrograms/ml). Inhibitors of medullasin such as phenylmethylsulfonyl fluoride and elastatinal prevented the activation of natural cytotoxicity. Depletion of lymphocytes bearing Fc receptors for IgG abolished the enhancement of natural killer cell activity by medullasin. Interferon activity was not detected in the supernatant of lymphocyte cultures stimulated with medullasin. The medullasin enhanced further the natural killer cell activity of lymphocytes stimulated with interferon. Medullasin activity was detected neither in unstimulated nor stimulated (by concanavalin A or phytohemagglutinin) human lymphocytes. The protease was released easily from human mature granulocytes into culture medium. It is considered from these results that the level of human natural killer cell activity is regulated by medullasin released by mature granulocytes.
大分子抗蛋白酶抑制人类天然细胞毒性。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hudig,D;Haverty,T;Fulcher,C;Redelman,D;Mendelsohn,J
通讯作者：
Mendelsohn,J
Tuftsin：一种天然存在的免疫增强因子。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Phillips,JH;Babcock,GF;Nishioka,K
通讯作者：
Nishioka,K