A dual switch controls bacterial enhancer-dependent transcription.
A dual switch controls bacterial enhancer-dependent transcription.
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DOI：
10.1093/nar/gks844
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发表时间：
2012-11
影响因子：
14.9
通讯作者：
Buck M
中科院分区：
文献类型：
--
作者：
Wiesler SC;Burrows PC;Buck M
细菌RNA聚合酶（RNAP）是抗生素的靶标。Myxopyronin与RNAP开关区域结合以阻断形成开放启动子复合物所需的结构重排。含有主要变体σ54因子的细菌RNAP被增强子结合蛋白（bEBP）激活并转录其产物在致病性和应激反应中所需的基因。我们发现，（i）增强子依赖的RNAP帮助大肠杆菌生存的存在下，myxopyronin，（ii）增强子依赖的RNAP部分抵抗抑制myxopyronin和（iii）ATP水解催化bEBPs是强制性的RNAP开关区域与转录起始位点的功能相互作用。我们证明，增强子依赖的启动子包含两个障碍，完全DNA开放，允许转录起始的严格调控。bEBP参与双重开关，以（i）允许从上游DNA叉连接处解链的有核DNA的传播，以及（ii）在RNA合成起始位点完成转录泡和下游DNA叉连接的形成，导致开关区域依赖性RNAP钳闭合和开放启动子复合物形成。
Bacterial RNA polymerases (RNAPs) are targets for antibiotics. Myxopyronin binds to the RNAP switch regions to block structural rearrangements needed for formation of open promoter complexes. Bacterial RNAPs containing the major variant σ54 factor are activated by enhancer-binding proteins (bEBPs) and transcribe genes whose products are needed in pathogenicity and stress responses. We show that (i) enhancer-dependent RNAPs help Escherichia coli to survive in the presence of myxopyronin, (ii) enhancer-dependent RNAPs partially resist inhibition by myxopyronin and (iii) ATP hydrolysis catalysed by bEBPs is obligatory for functional interaction of the RNAP switch regions with the transcription start site. We demonstrate that enhancer-dependent promoters contain two barriers to full DNA opening, allowing tight regulation of transcription initiation. bEBPs engage in a dual switch to (i) allow propagation of nucleated DNA melting from an upstream DNA fork junction and (ii) complete the formation of the transcription bubble and downstream DNA fork junction at the RNA synthesis start site, resulting in switch region-dependent RNAP clamp closure and open promoter complex formation.
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影响因子：
16
作者：
Bose, Daniel;Pape, Tillmann;Burrows, Patricia C.;Rappas, Mathieu;Wigneshweraraj, Siva R.;Buck, Martin;Zhang, Xiaodong
通讯作者：
Zhang, Xiaodong
影响因子：
64.5
作者：
Friedman LJ;Gelles J
通讯作者：
Gelles J
影响因子：
16
作者：
Kettenberger, H;Armache, KJ;Cramer, P
通讯作者：
Cramer, P
影响因子：
56.9
作者：
Cramer, P;Bushnell, DA;Kornberg, RD
通讯作者：
Kornberg, RD
DOI：
10.1073/pnas.1001188107
发表时间：
2010-05-18
影响因子：
11.1
作者：
Burrows, Patricia C.;Joly, Nicolas;Buck, Martin
通讯作者：
Buck, Martin