Proteomic analysis using protein chips to detect biomarkers in cervical and amniotic fluid in women with intra-amniotic inflammation
Proteomic analysis using protein chips to detect biomarkers in cervical and amniotic fluid in women with intra-amniotic inflammation
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DOI：
10.1021/pr050139e
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发表时间：
2005-11-01
影响因子：
4.4
通讯作者：
Jacobsson, B
中科院分区：
文献类型：
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作者：
Rüetschi, U;Rosén, Å;Jacobsson, B
羊膜内炎症(IAI)可导致早产，新生儿结局不良。为了确定新的IAI生物标志物，我们用表面增强激光解吸电离飞行时间质谱仪(SELDI-TOF-MS)分析了27例有或没有IAI威胁早产体征的患者的羊水和宫颈积液样本。17种蛋白在IAI患者的羊水中显著过表达，早产妇女高于胎膜破裂者。其中5个被鉴定为人中性粒细胞蛋白1-3、钙颗粒蛋白A和B。
Intra-amniotic inflammation (IAI) may cause preterm birth with poor neonatal out-come. To identify novel biomarkers for IAI, we analyzed amniotic and cervical fluid samples from 27 patients with signs of threatening preterm birth with or without IAI by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Seventeen proteins were significantly overexpressed in amniotic fluid from IAI cases and more often in women with preterm labor than those with rupture of membranes. Five of these were identified as human neutrophil protein 1-3, calgranulin A and B.