CNS innervation of vagal preganglionic neurons controlling peripheral airways: a transneuronal labeling study using pseudorabies virus
CNS innervation of vagal preganglionic neurons controlling peripheral airways: a transneuronal labeling study using pseudorabies virus
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DOI：
10.1016/s0165-1838(99)00020-x
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发表时间：
1999-05-28
期刊：
影响因子：
--
通讯作者：
Haxhiu, MA
中科院分区：
文献类型：
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作者：
Hadziefendic, S;Haxhiu, MA
通过病毒逆行跨神经元标记方法鉴定了投射到支配气道最远端的迷走神经节前神经元的CNS细胞群。将伪狂犬病病毒（PRV）注射到C8脊髓大鼠的肺实质中，存活5天后，对这些动物的脑组织切片进行免疫组织化学检测。逆行标记的副交感节前细胞（一级神经元）主要见于延髓腹侧：疑核的致密部及其腹侧区，偶尔也可在迷走背核的吻侧部见到少量标记细胞。这种标记模式与霍乱毒素β亚单位（CT-b）注射后在肺实质中观察到的逆行细胞体标记密切相关。PRV transneuronally标记的神经元（二级和/或假定的三级神经元）被发现在整个中枢神经系统与脑干的特征性标记。标记的神经元被确定为沿沿着和正下方的腹侧延髓表面，并在附近地区：旁锥体，后梯形，arctocellular和外侧paragigantocellular网状核，以及尾中缝核（中缝苍白，obscurus，和magnus）。几个核孤束（nTS）区域包含标记的细胞，包括连合，内侧，和腹外侧nTS亚核。A5细胞群和少量蓝斑神经元也被标记。PRV感染的神经元存在于Kolliker-Barrington核和Barrington核中。在中脑，腹侧脑室周围灰质内的神经元被标记。标记存在于下丘脑背侧核、外侧核和室旁核以及杏仁复合体内。总之，支配外周气道的副交感神经节前神经元由下脑干和桥上神经元的网络控制，所述下脑干和桥上神经元位于已知参与自主神经功能的中枢调节的相同区域。(C)1999年由Elsevier Science B. V.出版，版权所有。
The CNS cell groups that project to vagal preganglionic neurons which innervate the most distal part of the airways were identified by the viral retrograde transneuronal labeling method. Pseudorabies virus (PRV) was injected into the lung parenchyma of C8 spinal rats and after 5 days survival, brain tissue sections from these animals were processed for immunohistochemical detection of PRV. Retrogradely labeled parasympathetic preganglionic cells (first-order neurons) were seen mainly in the ventral medulla oblongata: the compact portion of the nucleus ambiguus and the area ventral to it. Occasionally, a few labeled cells were seen within the rostral part of the dorsal vagal nucleus. This labeling pattern correlated well with the retrograde cell body labeling seen following cholera toxin beta-subunit (CT-b) injections in the lung parenchyma. PRV transneuronally labeled neurons (second-order and/or presumed third-order neurons) were found throughout the CNS with the characteristic labeling in the brainstem. Labeled neurons were identified along and just beneath the ventral medullary surface, and in nearby areas: the parapyramidal, retrotrapezoid, gigantocellular and lateral paragigantocellular reticular nuclei, as well as the caudal raphe nuclei (raphe pallidus, obscurus, and magnus). Several nucleus tractus solitarius (nTS) regions contained labeled cells including the commissural, medial, and ventrolateral nTS subnuclei. The A5 cell group and a small number of locus coeruleus neurons were also labeled. PRV-infected neurons were present in the Kolliker-Fuse and Barrington's nuclei. In the mesencephalon, neurons within the ventral periventricular gray matter were labeled. Labeling was present in the dorsal, lateral and paraventricular hypothalamic nuclei, and within the amygdaloid complex. In summary, the parasympathetic preganglionic neurons that innervate the peripheral airways are controlled by networks of lower brainstem and suprapontine neurons that lie in the same regions known to be involved in central regulation of autonomic functions. (C) 1999 Published by Elsevier Science B.V. All rights reserved.