Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes
Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes
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DOI：
10.1111/j.1745-7262.2006.00125.x
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发表时间：
2006-05-01
影响因子：
2.9
通讯作者：
Hauschildt, S
中科院分区：
文献类型：
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作者：
Friedrich, M;Grahnert, A;Hauschildt, S
目的：目的探讨单腺苷二磷酸核糖基转移酶3（ART 3）mRNA的相应蛋白在人睾丸中是否有表达，如果有，其表达是否具有细胞类型特异性。方法：采用逆转录-聚合酶链反应（RT-PCR）技术检测人睾丸和精子中ART 3 mRNA的表达。通过用磷脂酶C处理ART 3转染的HEK-293-T细胞显示ART 3的糖基磷脂酰肌醇键。荧光激活细胞分选仪（FACS）分析用于检测成熟精子上的ART 3，免疫组织学研究用于检测睾丸中的蛋白质。结果：ART 3蛋白在睾丸组织中有表达。仅在精母细胞上发现。在精原细胞、精子细胞和精子中均不存在。通过FACS分析证实精子中不存在ART 3。ART 3蛋白既未在睾丸间质细胞内也未在睾丸间质细胞上检测到。结论：在这里，我们首次表明，ART 3蛋白表达在睾丸，特别是精母细胞，表明ART 3发挥特定的功能，只需要在精子发生的特定阶段。
Aim: To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 (ART3) mRNA is expressed in human testes and, if so, whether the expression is cell type-specific. Methods: ART3 mRNA was determined in human testes and sperm by reverse transcription-polymerase chain reaction (RT-PCR). The glycosylphosphatidylinositol linkage of ART3 was shown by treating ART3-transfected HEK-293-T cells with phospholipase C. Fluorescent activated cell sorter (FACS)-analyses were used to detect ART3 on mature spermatozoa and immunohistological studies to detect the protein in testes. Results: ART3 protein was shown to be present in testes. It was found on spermatocytes only. It was absent from spermatogonia, spermatids and spermatozoa. The absence of ART3 from spermatozoa was confirmed by FACS-analysis. ART3 protein was detected neither within a seminoma nor on Leydig cells. Conclusion: Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.