Systemic Nitric Oxide Production Rate during Hemodialysis and Its Relationship with Nitric Oxide-Related Factors
Systemic Nitric Oxide Production Rate during Hemodialysis and Its Relationship with Nitric Oxide-Related Factors
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DOI：
10.1159/000087769
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发表时间：
2005-09
影响因子：
3
通讯作者：
S. Mochizuki;Jun-ichi Ono;T. Yada;Y. Ogasawara;T. Miyasaka;M. Kimoto;N. Kashihara;F. Kajiya
中科院分区：
文献类型：
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作者：
S. Mochizuki;Jun-ichi Ono;T. Yada;Y. Ogasawara;T. Miyasaka;M. Kimoto;N. Kashihara;F. Kajiya
背景/目的：一氧化氮（NO）在调节血管张力和控制局部和全身血流动力学中起关键作用。在这里，我们估计血液透析（HD）患者的全身NO产生率，根据血浆硝酸盐（NO的氧化终产物）浓度的时间过程，并研究NO相关因素的可能作用。研究方法：我们测量了血浆中硝酸盐，L-精氨酸（一氧化氮合酶的底物：NOS），不对称二甲基精氨酸（ADMA，内源性NOS抑制剂），四氢生物蝶呤（BH 4，NOS辅因子），二氢生物蝶呤（BH 2，BH 4的氧化形式）和氧化低密度脂蛋白（氧化低密度脂蛋白;氧化应激指数）之前和之后30分钟和4小时HD（n = 10）。结果：血透期间血浆硝酸盐浓度随时间变化符合单室模型（4.00 ± 0.82 μmol/min，4.99 ± 1.08 μmol/kg/h），血透期间NO生成速率随时间变化符合单室模型（4.99 ± 1.08 μmol/kg/h）。HD 30 min后L-精氨酸/ADMA比值（L-精氨酸利用率）与NO生成率呈正相关（p < 0.05）。结论：单室分析法可用于血透时NO生成速率的估计。L-精氨酸/ADMA比例似乎在HD期间NO产生的调节中发挥重要作用。
Background/Aims:Nitric oxide (NO) plays a key role in the regulation of vascular tone and controls both local and systemic hemodynamics. Here, we estimated systemic NO production rates of hemodialysis (HD) patients, based on the time course of plasma concentration of nitrate (an oxidative end product of NO) and investigated possible roles of NO-related factors. Methods: We measured plasma concentrations of nitrate, L-arginine (a substrate of NO synthase: NOS), asymmetric dimethylarginine (ADMA, an endogenous NOS inhibitor), tetrahydrobiopterin (BH4, a NOS cofactor), dihydrobiopterin (BH2, an oxidized form of BH4) and oxidized low-density lipoprotein (oxyLDL; an index of oxidative stress) before and after 30-min and 4-hour HD (n = 10). Results:The time-averaged NO production rate during HD was estimated by fitting the time course of plasma nitrate concentration with a single-compartment model (4.00 ± 0.82 µmol/min, 4.99 ± 1.08 µmol/kg/h). The L-arginine/ADMA ratio (L-arginine availability) after 30-min HD showed a positive correlation with the NO production rate (p < 0.05). Conclusion: The systemic NO production rate during HD could be estimated by the single-compartment analysis. The L-arginine/ADMA ratio seems to play an important role in the regulation of the NO production during HD.