One-Step Surface Modification to Graft DNA Codes on Paper: The Method, Mechanism, and Its Application
One-Step Surface Modification to Graft DNA Codes on Paper: The Method, Mechanism, and Its Application
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DOI：
10.1021/acs.analchem.0c00317
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发表时间：
2020-05-19
影响因子：
7.4
通讯作者：
Li，Xiujun
中科院分区：
文献类型：
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作者：
Zhou，Wan;Feng，Mengli;Li，Xiujun
几十年来，载玻片已广泛用于DNA微阵列和众多生物测定中的DNA固定，但它们面临着探针密度低、修饰步骤耗时和仪器昂贵的限制。在这项工作中，一个简单的一步表面改性方法，使用3-氨丙基三甲氧基硅烷（APTMS）已被开发和应用于接枝DNA代码在纸上。与使用载玻片的常规方法相比，获得了更高的DNA固定效率。荧光检测，X射线光电子能谱（XPS），红外光谱（FT-IR），和pH值的影响的研究进行了表征的表面修饰和随后的DNA固定，这进一步揭示了一种机制，其中该方法在于带正电荷的APTMS修饰的纸张表面和带负电荷的DNA探针之间的离子相互作用。此外，APTMS改良的纸基设备已被开发，以证明在低成本检测食源性病原体，贾第鞭毛虫，具有高灵敏度（检测限为22 nM）和高特异性的应用。与使用冗余交联反应的传统方法相比，我们的方法更简单，更快，通用性更强，成本更低，使纸基生物测定的广泛应用，特别是在资源匮乏的环境中进行即时检测。
Glass slides have been widely used for DNA immobilization in DNA microarray and numerous bioassays for decades, whereas they are faced with limitations of low probe density, time-consuming modification steps, and expensive instruments. In this work, a simple one-step surface modification method using 3-aminopropyl trimethoxysilane (APTMS) has been developed and applied to graft DNA codes on paper. Higher DNA immobilization efficiency was obtained in comparison with that in a conventional method using glass slides. Fluorescence detection, X-ray photoelectron spectroscopy (XPS), infrared spectra (FT-IR), and pH influence studies were employed to characterize the surface modification and subsequent DNA immobilization, which further reveals a mechanism in which this method lies in ionic interactions between the positively charged APTMS-modified paper surface and negatively charged DNA probes. Furthermore, an APTMS-modified paper-based device has been developed to demonstrate application in low-cost detection of a foodborne pathogen,Giardia lamblia, with high sensitivity (the detection limit of 22 nM) and high specificity. Compared with conventional methods using redundant cross-linking reactions, our method is simpler, faster, versatile, and lower-cost, enabling broad applications of paper-based bioassays especially for point-of-care detection in resource-poor settings.