Evaluation of a Lyme disease enzyme immunoassay using the 41-G fragment of flagellin.
Evaluation of a Lyme disease enzyme immunoassay using the 41-G fragment of flagellin.
复制标题
使用鞭毛蛋白 41-G 片段评估莱姆病酶免疫测定。
DOI：
10.1007/bf01690736
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Fikrig,E
中科院分区：
文献类型：
--
作者：
Cretella,S;Gordon,S;Flavell,RA;Fikrig,E
在一个参比实验室中，对伯氏疏螺旋体鞭毛蛋白的免疫原性区域（包括氨基酸197-273和指定41-G）作为莱姆病的酶免疫测定（EIA）抗原进行了常规评估。采用41-G或全细胞伯氏疏螺旋体溶酶解液作为抗原，经EIA检测为莱姆病阳性的血清也通过免疫印迹法评估伯氏疏螺旋体的反应性，并通过向转诊医生分发问卷的方式回顾性确定患者的临床病史。与伯氏疏螺旋体的EIA相比，基于41-G的EIA对莱姆病血清学诊断的敏感性为70%（35 / 50）。这些数据表明，41 -G在EIA中具有作为抗原的效用，尽管目前的敏感性低于采用伯氏疏螺旋体全细胞裂解物的检测。
An immunogenic region of theBorrelia burgdorferiflagellin encompassing amino acids 197–273 and designated 41-G was evaluated as an antigen in an enzyme immunoassay (EIA) for Lyme disease on a routine basis in a reference laboratory. Sera that tested positive for Lyme disease by EIA using 41-G or the whole-cellBorrelia burgdorferilysate as the antigen were also evaluated by immunoblot for reactivity withBorrelia burgdorferi, and the patient's clinical history was determined retrospectively by a questionnaire distributed to the referring physician. The sensitivity of the 41-G based EIA for the serologic diagnosis of Lyme disease, when compared with that of theBorrelia burgdorferilysate EIA, was 70 % (35 of 50). These data demonstrate that 41 -G has utility as an antigen in EIA, although the sensitivity is at present less than that of the assay employing theBorrelia burgdorferiwhole-cell lysate.