Extraction of flavonoids from Tagetes patula: process optimization and screening for biological activity
Extraction of flavonoids from Tagetes patula: process optimization and screening for biological activity
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DOI：
10.1016/j.bjp.2014.10.001
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发表时间：
2014-10-01
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通讯作者：
Mello, João C.P.
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作者：
Munhoz, Vanessa M.;Longhini, Renata;Mello, João C.P.
万寿菊，菊科万寿菊，俗称法国万寿菊，在民间医药中用作杀菌、利尿、净化血液和驱虫。本研究以总黄酮类化合物的含量为指标，采用统计混合单纯形-质心设计，以水、乙醇、丙酮为溶剂，以总黄酮含量为指标，对黄酮类化合物的提取工艺进行了优化。对提取物的干残留物、清除自由基活性、层析图谱和杀幼虫活性进行了测试。丙酮提取物的总黄酮含量最高，为25.13+/-1.02%(4.07%)，清除自由基活性最强，IC50为15.74µg/ml+/-1.09(6.92%)，干残留量较低，为6.62+/-1.33%(20.10%)。水提物干残留量高于丙酮提物，但总黄酮含量和清除自由基活性低于丙酮提物。提取物中总黄酮含量与清除自由基活性呈正相关，表明黄酮类化合物对抗氧化能力有显著的贡献。采用统计混合设计，以丙酮为最佳提取溶剂，优化了百草花中黄酮类化合物的提取工艺。初步的生物活性研究表明，丙酮提取物对埃及伊蚊有一定的杀灭作用。(C)2014年巴西农牧业银行。书名/作者Elsevier Editora Ltd.版权所有。
The flowers of Tagetes patula L., Asteraceae, commonly known as French marigold, are used in folk medicine as an antiseptic, diuretic, blood purifier and insect repellent. This study was conducted to optimize the extraction process through the biomonitoring of flavonoids, using a statistical mixture simplex-centroid design, to evaluate the effect of the solvents water, ethanol and acetone, as well as mixtures of these solvents, assessed by the total flavonoid content. The extracts were tested for dry residue, radical scavenging activity, chromatographic profile, and larvicidal activity. The acetone extract had the highest total flavonoid content, 25.13 +/- 1.02% (4.07%); and the best radical scavenging activity, with IC50 of 15.74 mu g/ml +/- 1.09 (6.92%), but with lower dry residue, 6.62 +/- 1.33% (20.10%). The water extracts showed higher levels of dry residue, but lower total flavonoid content and radical scavenging activity than the acetone extract. The positive correlation between the total flavonoid content and radical scavenging activity of the extracts showed that flavonoids contribute significantly to the antioxidant capacity. The statistical mixture design allowed us to optimize the extraction of flavonoids from flowers of T. patula, with acetone as the best extraction solvent. Preliminary studies on the biological activity of the optimized extracts demonstrated a larvicidal effect of the acetone extract on Aedes aegypti mosquitoes. (C) 2014 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved.