Correlation of Rhinovirus Load in the Respiratory Tract and Clinical Symptoms in Hospitalized Immunocompetent and Immunocompromised Patients
Correlation of Rhinovirus Load in the Respiratory Tract and Clinical Symptoms in Hospitalized Immunocompetent and Immunocompromised Patients
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DOI：
10.1002/jmv.21548
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发表时间：
2009-08-01
影响因子：
12.7
通讯作者：
Meloni, F.
中科院分区：
文献类型：
--
作者：
Gerna, G.;Piralla, A.;Meloni, F.
虽然人鼻病毒（HRV）被公认为是普通感冒综合征（鼻炎）的主要原因，但其在下呼吸道感染病因学中的作用仍有争议，并且在无症状患者中检测到它们相对常见。通过实时逆转录聚合酶链反应（RT-PCR）定量鼻咽抽吸物或支气管肺泡灌洗液样本中的HRV负荷，研究了四组住院患者（儿童免疫功能正常和免疫功能低下患者，成人免疫功能正常和免疫功能低下患者）中HRV的致病作用。对通过定性RT-PCR得到阳性结果的所有呼吸道样本进行一式两份实时RT-PCR。此外，分子分型允许检测所有已知的HRV种类（A、B和Q）。在免疫功能正常的儿科患者中，当病毒载量>= 10（6）RNA拷贝/ml时，HRV主要与下呼吸道感染（在没有其他病毒因子的情况下）和喘息相关。在年轻的免疫功能低下患者（干细胞移植受者）中，观察到HRV持续时间与移植后发生感染的时间之间呈负相关，而在成年免疫功能低下患者（肺移植受者）中，可以检测到中等-低水平的HRV（
While human rhinoviruses (HRVs) are well accepted as a major cause of common cold syndromes (rhinitis), their role in the etiology of lower respiratory tract infections is still controversial, and their detection in asymptomatic patients is relatively common. The HRV pathogenic role in four groups of hospitalized patients (pediatric immunocompetent and immunocompromised patients, and adult immunocompetent and immunocompromised patients) was investigated by quantifying HRV load in nasopharyngeal aspirates or bronchoalveolar lavage samples by real-time reverse transcription PCR (RT-PCR). Real-time RT-PCR was performed in duplicate on all respiratory samples resulting positive by qualitative RT-PCR. In addition, molecular typing allowed detection of all known HRV species (A, B, and Q. In immunocompetent pediatric patients HRVs were mostly associated with lower respiratory tract infections (in the absence of other viral agents) and wheezing, when viral load was >= 10(6) RNA copies/ml. In young immunocompromised patients (stem cell transplantation recipients), an inverse correlation between HRV persistence overtime and time at which the infection occurred after transplantation was observed, whereas in adult immunocompromised patients (lung transplant recipients) HRVs could be detected at a medium-low level (