MoImd4 mediates crosstalk between MoPdeH-cAMP signalling and purine metabolism to govern growth and pathogenicity in Magnaporthe oryzae
MoImd4 mediates crosstalk between MoPdeH-cAMP signalling and purine metabolism to govern growth and pathogenicity in Magnaporthe oryzae
复制标题
MoImd4 介导 MoPdeH-cAMP 信号传导和嘌呤代谢之间的串扰，以控制稻瘟病菌的生长和致病性
DOI：
10.1111/mpp.12770
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发表时间：
2019
影响因子：
4.9
通讯作者：
Zhang Zhengguang
中科院分区：
文献类型：
--
作者：
Yang Lina;Ru Yanyan;Cai Xingjia;Yin Ziyi;Liu Xinyu;Xiao Yuhan;Zhang Haifeng;Zheng Xiaobo;Wang Ping;Zhang Zhengguang
The high‐affinity cyclic adenosine monophosphate (cAMP) phosphodiesterase MoPdeH is important not only for cAMP signalling and pathogenicity, but also for cell wall integrity (CWI) maintenance in the rice blast fungusMagnaporthe oryzae. To explore the underlying mechanism, we identified MoImd4 as an inosine‐5′‐monophosphate dehydrogenase (IMPDH) homologue that interacts with MoPdeH. Targeted deletion ofMoIMD4resulted in reducedde novopurine biosynthesis and growth, as well as attenuated pathogenicity, which were suppressed by exogenous xanthosine monophosphate (XMP). Treatment with mycophenolic acid (MPA), which specifically inhibits MoImd4 activity, resulted in reduced growth and virulence attenuation. Intriguingly, further analysis showed that MoImd4 promotes the phosphodiesterase activity of MoPdeH, thereby decreasing intracellular cAMP levels, and MoPdeH also promotes the IMPDH activity of MoImd4. Our studies revealed the presence of a novel crosstalk between cAMP regulation and purine biosynthesis inM. oryzae, and indicated that such a link is also important in the pathogenesis ofM. oryzae.
