Sleep and Michaelis‐Menten elimination of ethanol
Sleep and Michaelis‐Menten elimination of ethanol
复制标题
睡眠和米氏消除乙醇
DOI：
10.1038/clpt.1980.17
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发表时间：
1980
影响因子：
6.7
通讯作者：
Lorena Rossi
中科院分区：
文献类型：
--
作者：
B. Madsen;Lorena Rossi
6名健康空腹志愿者(3男3女)在2300hr分4次口服酒精(0.6gm/kg)作为鸡尾酒。受试者两次卧床保持清醒，另两次允许睡眠。采用前臂静脉留置导管在不干扰睡眠的情况下采血，并在鸡尾酒后8小时内适当间隔抽取20份连续血样(2ml)。用顶空气相色谱技术测定血液中乙醇浓度，并根据末次消除阶段的数据估算米氏参数(Vm和Km)。平均估算为Vm清醒=0.214±0.009 mg/ml/小时(扫描电子显微镜)，Vm睡眠=0.221±0.017，Km清醒=0.069±0.009 mg/ml，Km睡眠=0.085±0.016。这些数值略低于文献报道的白天非卧床患者的消除。Vm和Km在清醒组和睡眠组之间以及性别之间的平均差异均不显著(p>；0.05)。
Ethanol (0.6 gm/kg) was given orally as a cocktail to 6 healthy fasted volunteers (3 men and 3 women) on 4 separate occasions at 2300 hr. Subjects were recumbent and remained awake in 2 sessions while sleep was allowed in the other 2. An indwelling forearm venous catheter was used to allow blood sampling without disturbance of sleep, and 20 serial blood samples (2 ml) were drawn at appropriate intervals in the 8 hr after the cocktail. Blood ethanol concentration was determined by a head‐space gas chromatographic technique and Michaelis‐Menten parameters (Vm and Km) were estimated from data in the terminal elimination phase. Mean estimates were Vm awake = 0.214 ± 0.009 mg/ml/hr (SEM), Vm asleep = 0.221 ± 0.017, Km awake = 0.069 ± 0.009 mg/ml, and Km asleep = 0.085 ± 0.016. These values were slightly lower than literature reports for elimination in ambulant patients during the daytime. Mean differences in Vm and Km between the awake and asleep groups and between sexes were not significant (p > 0.05).