Rises in antibody to human herpesvirus 6 detected by enzyme immunoassay in transplant recipients with primary cytomegalovirus infection
Rises in antibody to human herpesvirus 6 detected by enzyme immunoassay in transplant recipients with primary cytomegalovirus infection
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原发性巨细胞病毒感染移植受者体内酶联免疫分析检测到人疱疹病毒 6 型抗体升高
DOI：
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发表时间：
1990
影响因子：
9.4
通讯作者：
K. Scott
中科院分区：
文献类型：
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作者：
S. Chou;K. Scott
用双抗体夹心法检测实体器官移植前后血清中抗人疱疹病毒6型(HHV-6)和巨细胞病毒(CMV)的免疫球蛋白G。用HHV-6感染和未感染HSB-2细胞的甘氨酸提取液建立了HHV-6酶联免疫吸附试验。在血清稀释度为1：500时，88名受者中有80名(91%)移植前HHV-6血清阳性，而只有14名(16%)CMV血清阳性。移植后观察到38名(43%)受者HHV-6血清学升高；其中25名受者与原发CMV感染有关。血清滴度检测显示，原发CMV感染者的HHV-6滴度升幅明显高于CMV复活者和无CMV感染者。在初次感染巨细胞病毒后，HHV-6抗体滴度升高持续长达2年之久。在个体血清样本中，CMV和HHV-6抗体效价之间没有相关性。
Immunoglobulin G to human herpesvirus 6 (HHV-6) and cytomegalovirus (CMV) in sera from solid organ recipients was measured by an enzyme-linked immunoassay (ELISA) before and after transplant. The HHV-6 ELISA was developed from glycine extracts of HHV-6-infected and uninfected HSB-2 cells. At a serum dilution of 1:500, 80 (91%) of 88 recipients were seropositive for HHV-6 before transplant, while only 14 (16%) were seropositive for CMV. Posttransplant HHV-6 serologic rises were observed in 38 (43%) recipients; rises in 25 of these recipients were associated with primary CMV infection. Titration of sera revealed much higher HHV-6 titer rises among those with primary CMV infection than among those with CMV reactivation or with no CMV infection. Elevated HHV-6 antibody titers persisted for up to 2 years after primary CMV infection. No correlation was noted between CMV and HHV-6 antibody titers in individual serum samples.