Identification of the s E regulon of Salmonella enterica serovar Typhimurium
Identification of the s E regulon of Salmonella enterica serovar Typhimurium
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鼠伤寒沙门氏菌血清E调节子的鉴定
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
A. Kralli
中科院分区：
文献类型：
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作者：
M. Gantner;Bethany C. Hazen;J. Conkright;A. Kralli
细胞浆外功能sigma因子s已被证明在肠炎沙门氏菌血清型鼠伤寒沙门氏菌（s . Typhimurium）的毒力中起关键作用。先前优化的双质粒系统已用于鉴定含有s的RNA聚合酶识别的鼠伤寒沙门氏菌启动子。该方法鉴定了34个s依赖性启动子，这些启动子在鼠伤寒沙门氏菌中直接表达62个基因，其中23个（包括鼠伤寒沙门氏菌特异性的几个）以前未被鉴定为依赖于大肠杆菌中的s。在鼠伤寒沙门氏菌中确认了启动子，并通过s1 -核酸酶作图确定了启动子的转录起始点。所有启动子都包含与s依赖启动子的一致序列高度相似的序列。所鉴定的鼠伤寒沙门氏菌s-调节子基因编码参与初级代谢、DNA修复系统和外膜生物发生的蛋白，以及调节蛋白、质周蛋白酶和折叠因子、拟制脂蛋白和功能未知的内外膜蛋白。其中一些依赖s的基因已被证明在鼠伤寒沙门氏菌的毒力中起作用。
Received 5 December 2005 Revised 20 January 2006 Accepted 30 January 2006 The extracytoplasmic function sigma factor, s, has been shown to play a critical role in virulence of Salmonella enterica serovar Typhimurium (S. Typhimurium). The previously optimized two-plasmid system has been used to identify S. Typhimurium promoters recognized by RNA polymerase containing s. This method allowed identification of 34 s-dependent promoters that direct expression of 62 genes in S. Typhimurium, 23 of which (including several specific for S. Typhimurium) have not been identified previously to be dependent upon s in Escherichia coli. The promoters were confirmed in S. Typhimurium and transcriptional start points of the promoters were determined by S1-nuclease mapping. All the promoters contained sequences highly similar to the consensus sequence of s-dependent promoters. The identified genes belonging to the S. Typhimurium s-regulon encode proteins involved in primary metabolism, DNA repair systems and outer-membrane biogenesis, and regulatory proteins, periplasmic proteases and folding factors, proposed lipoproteins, and innerand outer-membrane proteins with unknown functions. Several of these s-dependent genes have been shown to play a role in virulence of S. Typhimurium.
从葡萄糖到某些次级碳源的转变导致鼠伤寒沙门氏菌中胞质外功能 sigma 因子 sigmaE 的激活。
DOI：
10.1099/mic.0.27649-0
发表时间：
2005
期刊：
Microbiology (Reading, England)
影响因子：
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作者：
Kenyon,WilliamJ;Thomas,SheenaM;Johnson,Erin;Pallen,MarkJ;Spector,MichaelP
通讯作者：
Spector,MichaelP
大肠杆菌 K-12 蛋白酶 III (ptr) 基因调控区分析。
影响因子：
3.5
作者：
Claverie-Martin,F;Diaz-Torres,MR;Kushner,SR
通讯作者：
Kushner,SR
DOI：
10.1073/pnas.0308747101
发表时间：
2004-03-02
影响因子：
11.1
作者：
Bernstein, JA;Lin, PH;Lin-Chao, S
通讯作者：
Lin-Chao, S