Comparisons of tissue-specific transcription of stress response genes with whole animal endpoints of adverse effect in striped bass (Morone saxatilis) following treatment with copper and esfenvalerate
Comparisons of tissue-specific transcription of stress response genes with whole animal endpoints of adverse effect in striped bass (Morone saxatilis) following treatment with copper and esfenvalerate
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DOI：
10.1016/j.aquatox.2007.07.011
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发表时间：
2007-11-15
影响因子：
4.5
通讯作者：
Leutenegger, Christian M.
中科院分区：
文献类型：
--
作者：
Geist, Juergen;Werner, Inge;Leutenegger, Christian M.
留居鱼类应激反应基因转录的变化可以作为鉴定环境应激物对水生态系统亚致死影响的有力生物标志物。在这项研究中，我们测试了两种参考毒物，铜（Cu）和拟除虫菊酯类杀虫剂高氰戊菊酯[（S）-α-氰基-3-苯氧基苄基-（S）-2-（4-氯苯基）-3-甲基丁酸酯]，对致死（死亡率）和亚致死终点（生长，游泳行为，应激反应基因的转录水平）在青少年（81-90日龄）条纹鲈鱼（Morone saxatilis）。我们建立了蛋白毒性（HSP 70，HSP 90），I相解毒机制（CYP 1A 1），金属结合（金属硫蛋白），以及免疫功能和病原体防御（TGF-β，Mx蛋白，nRAMP）的细胞应激反应标志物。采用实时定量TaqMan（R）PCR检测了铜暴露7天和高氰戊菊酯暴露24小时后肝脏、脾脏、白色肌、前肾和鳃转录组的组织特异性变化。在转录组水平上，暴露于Cu对脾组织中金属硫蛋白的转录表现出最强的影响，导致mRNA在42 ppb总Cu时增加4倍，在160 ppb Cu时增加10倍。暴露于Cu也引起免疫系统相关基因的基因转录的显着组织特异性变化。高氰戊菊酯暴露对HSP 70、HSP 90和CYP 1A 1的转录具有组织特异性影响。暴露于0.64 μ g/L的esfenvalerate.Our结果表明，在转录组水平上的应激反应是一个更敏感的指标，铜和esfenvalerate. Our暴露在低浓度比游泳行为，生长或死亡后，在肝组织中检测到最显着的影响。转录组定量变化研究的准确性可以从初始评估或包括几种不同的组织和使用多个管家基因中受益。(c)2007 Elsevier B. V.保留所有权利。
Changes in the gene transcription of stress response genes in resident fish can be powerful biomarkers for the identification of sublethal impacts of environmental stressors on aquatic ecosystems. In this study, we tested the effects of two reference toxicants, copper (Cu) and the pyrethroid insecticide esfenvalerate [(S)-alpha-cyano-3-phenoxybenzyl-(S)-2-(4-chlorophenyl)-3-methylbutyrate], on lethal (mortality) and sublethal endpoints (growth, swimming behavior, transcription levels of stress response genes) in juvenile (81-90-day-old) striped bass (Morone saxatilis). We established cellular stress response markers for proteotoxicity (HSP70, HSP90), phase I detoxification mechanism (CYP1A1), metal-binding (metallothionein), as well as immune-function and pathogen-defense (TGF-beta, Mx-protein, nRAMP). Quantitative real-time TaqMan (R) PCR was used to examine tissue-specific changes in the transcriptome of liver, spleen, white muscle, anterior kidney and gills after 7-day Cu exposures and 24-h esfenvalerate exposures. On the transcriptome level, exposure to Cu showed strongest effects on the transcription of metallothionein in spleen tissue, causing a 4-fold increase of mRNA at 42 ppb total Cu and a 10-fold increase at 160 ppb Cu. Exposure to Cu also caused significant tissue-specific changes in gene transcription for immune-system related genes. Esfenvalerate exposure had tissue-specific effects on the transcription of HSP70, HSP90 and CYP1A1. The most significant effects were detected in liver tissue after exposure to 0.64 mu g/L esfenvalerate.Our results show that the stress response at the transcriptome level is a more sensitive indicator for Cu and esfenvalerate exposures at low concentrations than swimming behavior, growth or mortality. The accuracy of studies on quantitative changes in the transcriptome can benefit from an initial evaluation or the inclusion of several different tissues and the use of multiple housekeeping genes. (c) 2007 Elsevier B.V. All rights reserved.