Neurotransmitter properties of spinal interneurons in embryonic and larval zebrafish
Neurotransmitter properties of spinal interneurons in embryonic and larval zebrafish
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DOI：
10.1002/cne.20279
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发表时间：
2004-11-29
影响因子：
2.5
通讯作者：
Fetcho, JR
中科院分区：
文献类型：
--
作者：
Higashijima, S;Schaefer, M;Fetcho, JR
斑马鱼中许多种类的脊髓中间神经元已经根据形态学进行了描述，但它们的递质表型在很大程度上是未知的。在这里，我们将脊髓中间神经元的回填或遗传标记与神经递质表型标记的原位染色相结合，包括谷氨酸能神经元的囊泡谷氨酸转运蛋白（VGLUT）基因，甘氨酸能神经元的神经元甘氨酸转运蛋白（GLYT2）和gaba能神经元的谷氨酸脱羧酶（GAD）。VGLUT阳性的神经元包括交联CoPA、MCoD、UCoD和部分CoSA神经元。具有同侧下行轴突的CiD中间神经元也呈vglut阳性，位于腹侧的VeMe中间神经元也呈vglut阳性，其下行轴突轨迹尚未清楚显示。GLYT2阳性的细胞包括联合CoLAs以及一些CoBL和CoSA神经元。CiA细胞是唯一具有同侧轴突的glyt2阳性细胞。最明显的是，GAD染色细胞包括背纵向上升（DoLA）和KA中间神经元。我们的方法使我们能够定义大多数已知类型的脊髓中间神经元的可能递质表型。这些数据为了解斑马鱼脊髓网络的功能组织提供了基础。(C) 2004 Wiley-Liss, Inc。
Many classes of spinal interneurons in zebrafish have been described based on morphology, but their transmitter phenotypes are largely unknown. Here we combine back-filling or genetic labeling of spinal interneurons with in situ staining for markers of neurotransmitter phenotypes, including the vesicular glutamate transporter (VGLUT) genes for glutamatergic neurons, the neuronal glycine transporter (GLYT2) for glycinergic neurons, and glutamic acid decarboxylase (GAD) for GABAergic neurons. Neurons positive for VGLUT include the commissural CoPA, MCoD, UCoD, and some of the CoSA neurons. The CiD interneurons, which have ipsilateral descending axons, were also VGLUT-positive, as were the ventrally located VeMe interneurons, whose descending axonal trajectory has not been clearly revealed. Cells positive for GLYT2 include the commissural CoLAs as well as some of the CoBL and CoSA neurons. The CiA cells were the only GLYT2-positive cells with an ipsilateral axon. Cells staining for GAD included, most notably, the dorsal longitudinal ascending (DoLA) and KA interneurons. Our approach allowed us to define the likely transmitter phenotypes of most of the known classes of spinal interneurons. These data provide a foundation for understanding the functional organization of the spinal networks in zebrafish. (C) 2004 Wiley-Liss, Inc.