Predictive models for growth of Salmonella typhimurium DT104 from low and high initial density on ground chicken with a natural microflora.
Predictive models for growth of Salmonella typhimurium DT104 from low and high initial density on ground chicken with a natural microflora.
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DOI：
10.1016/j.fm.2006.11.003
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发表时间：
2007-09
影响因子：
5.3
通讯作者：
T. Oscar
中科院分区：
文献类型：
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作者：
T. Oscar
使用鼠伤寒沙门氏菌DT 104的单一菌株（ATCC 700408）研究和模拟具有天然微生物区系的地面鸡肉上低（1.12log10mpng−1）和高（3.7log10cfug−1）初始密度的生长。将病原体在地面鸡上生长的动力学数据拟合到初级模型以确定滞后时间（λ）、最大比生长速率（μ）和最大种群密度（Nmax）。建立了λ、μ和Nmax随温度（10-40°C）变化的二级模型，并在初始密度之间进行了比较。在10-18°C时，病原菌生长量的变异性大于22-40°C时的变异性，Nmax的变异性大于λ和μ。当用一个初始密度开发的二级模型从另一个初始密度预测λ、μ和Nmax时，观察到了预测问题，特别是在10-18°C和Nmax时。这些结果表明，重复挑战研究之间的生长变化和初始密度是建立S生长预测模型时要考虑的重要因素。鼠伤寒沙门氏菌DT 104在具有天然微生物区系的鸡肉地面上的应用。
A single strain (ATCC 700408) of Salmonella typhimurium DT104 was used to investigate and model growth from a low (1.12log10mpng−1) and high (3.7log10cfug−1) initial density on ground chicken with a natural microflora. Kinetic data for growth of the pathogen on ground chicken were fit to a primary model to determine lag time (λ), maximum specific growth rate (μ) and maximum population density (Nmax). Secondary models for λ, μ and Nmax, as a function of temperature (10–40°C), were developed and compared among initial densities. Variation of pathogen growth among replicates (n=4 or 5) was higher at 10–18°C than at 22–40°C and was higher for Nmaxthan λ and μ. Prediction problems were observed when secondary models developed with one initial density were used to predict λ, μ and Nmaxfrom the other initial density, especially at 10–18°C and for Nmax. These results indicated that variation of growth among replicate challenge studies and initial density are important factors to consider when developing predictive models for growth of S. typhimurium DT104 on ground chicken with a natural microflora.