Simple applications of microparticle transportation by tender optical scattering force
Simple applications of microparticle transportation by tender optical scattering force
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DOI：
10.1007/s10404-014-1459-y
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发表时间：
2015-04
影响因子：
2.8
通讯作者：
Hideharu Kotari;M. Motosuke
中科院分区：
文献类型：
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作者：
Hideharu Kotari;M. Motosuke
本文提供了一种新的应用于微流控粒子传输的光辐射压力，而不需要激光光束精确聚焦或对准装置和靶。微流控平台中的颗粒的光学操纵在生命科学或生物医学分析中被高度利用，该光学操纵使用光学镊子，利用光学辐射压力的梯度力。我们的方法利用辐射压力的另一项，即散射力，来操纵微通道中的粒子。粒子的迁移距离取决于粒子接收的光量。因此，即使在较低的能量密度下，也可以通过大面积辐照实现长保留距离的粒子运动。在我们的实验中，我们设计并研究了两种概念验证微流控芯片：一种是使用集成了SU-8平板波导和扩束器的整体式微流控芯片进行横向粒子分选，另一种是使用10厘米长的聚二甲基硅氧烷沟道进行垂直分选，并进行了大面积辐照。实验结果表明，1、2和5μm聚苯乙烯微珠可以通过光散射力传输，在辐照能量密度为10 mW/mm~2的情况下实现粒子迁移。这种方法具有实用的潜力，可以简单和自由地使用光辐射压力，而不需要激光光束的光斑聚焦或精确对准过程，也不会对器件和样品造成损害。
This paper provides a novel application of the optical radiation pressure for microfluidic particle transportation without precise focusing or alignment of the laser beam to the device and target. An optical manipulation of particles in a microfluidic platform is highly exploited in life science or biomedical analysis using optical tweezers with the use of a gradient force of the optical radiation pressure. Our method utilizes the other term of the radiation pressure, namely scattering force, to manipulate particles in a microchannel. The migration distance of particle depends on the amount of light received by the particle. Therefore, particle movement with long retention distance can be achieved by large-area irradiation even with low energy density. In our experiments, two proof-of-concept microfluidic chips were designed and investigated; one was a lateral particle sorting using a monolithic microfluidic chip integrated with a planar SU-8 waveguide and beam expander, the other was a vertical sorting using a 10-cm-long polydimethylsiloxane channel with whole-area irradiation. Experimental results show that 1, 2 and 5 μm polystyrene beads can be transported by the optical scattering force and that particle migration is achieved with the irradiated energy density <10 mW/mm2. The present method has practical potential for simple and fuss-free use of the optical radiation pressure without spot focusing or precise alignment process of the laser beam and damage to the device and sample.