Sini Tang Prevents Depression-Like Behavior in Rats Exposed to Chronic Unpredictable Stress
Sini Tang Prevents Depression-Like Behavior in Rats Exposed to Chronic Unpredictable Stress
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四逆汤可预防慢性不可预测压力下的大鼠出现抑郁样行为
DOI：
10.1142/s0192415x0900693x
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发表时间：
2009-01-01
影响因子：
5.7
通讯作者：
Jiang, Ting-Liang
中科院分区：
文献类型：
--
作者：
Guo, Jian-You;Huo, Hai-Ru;Jiang, Ting-Liang
四逆汤是一种含有三种草药的传统中药，被广泛用于治疗阳虚。近年来的临床研究表明，四逆汤可以治疗和改善抑郁症状，但其抗抑郁作用的机制尚不清楚。在慢性不可预测应激大鼠（CUS）中，我们研究了四逆汤对蔗糖偏好和开阔地探索行为的影响。分别采用酶联免疫吸附法（ELISA）和实时反转录PCR法（RT-PCR）检测大鼠血浆皮质酮水平和下丘脑促肾上腺皮质激素释放激素（CRH） mRNA表达。在野外实验中，大鼠对蔗糖的偏好和行动能力均有所下降。四逆汤均呈剂量依赖性减毒。从生化角度看，四逆汤还能逆转cuss诱导的血浆皮质酮和下丘脑CRH mRNA的升高。四逆汤的行为效应与生化作用相关。这些结果表明，四逆汤具有类似抗抑郁的作用，似乎与大脑中的CRH有关。
Sini Tang, a Chinese traditional prescription containing three herbs, has been widely used for Yang-deficiency. Recent clinical studies have shown that Sini Tang could treat and improve depression symptoms, but the mechanisms underlying the antidepressant effect of Sini Tang remains unknown. In rats with chronic unpredictable stress (CUS), we examined the effects of Sini Tang on sucrose preference and open field exploratory behavior. The levels of corticosterone level in plasma and corticotropin-releasing hormone (CRH) mRNA expression in hypothalamus were also measured by enzyme-linked immunosorbent assays (ELISA) and real-time reverse transcription PCR (RT-PCR), respectively. Rats subjected to CUS exhibited decreases in sucrose preference and ambulation in the open field test. These were all attenuated by Sini Tang in a dose-dependent manner. Biochemically, Sini Tang also reversed CUS-induced increases in corticosterone in plasma and CRH mRNA in the hypothalamus. The behavioral effects of the Sini Tang were correlated to the biochemical actions. These results suggest that Sini Tang produces an antidepressant-like effect, which appears to involve CRH in the brain.