Chitosan-based particles for protection of proteins during storage and oral administration
Chitosan-based particles for protection of proteins during storage and oral administration
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用于在储存和口服给药过程中保护蛋白质的壳聚糖颗粒
DOI：
10.1016/j.ijbiomac.2018.05.208
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发表时间：
2018
影响因子：
8.2
通讯作者：
Yan Liang
中科院分区：
文献类型：
--
作者：
Wenbei Situ;Tuo Xiang;Yan Liang
交联壳聚糖颗粒已被开发用于改善生物活性蛋白的储存稳定性，并通过靶向释放到结肠来确保足够的生物利用度。使用小角X射线散射、X射线电位、粒径和形态证明了这些使用不同分子量壳聚糖和不同阴离子交联剂形成的基于壳聚糖的颗粒的特性。体外释药研究表明，在上消化道中，蛋白质从颗粒中释放<30%，提示靶向结肠释放的可能性。一些颗粒显示在PBS缓冲液中储存后保留结肠靶向蛋白质递送。通过控制壳聚糖基颗粒的结构，可以减少短期储存期间的蛋白质泄漏并实现结肠靶向递送。
Crosslinked chitosan particles have been developed to improve the storage stability of bioactive proteins and to ensure adequate bioavailability by targeting release to the colon. The characteristics of these chitosan-based particles, formed using different molecular weight chitosans and different anion cross-linkers, were demonstrated using small angle X-ray scattering, ζ-potential, particle size, and morphology. Anin vitrorelease study showed that protein release from the particles in the upper gastrointestinal tract was <30%, suggesting the possibility for target release to the colon. Some of the particles were shown to retain colon-targeted protein delivery after storage in PBS buffer. By controlling the structure of the chitosan-based particles, it was possible to both reduce protein leakage during short-term storage and achieve colon-targeted delivery.