Oligonucleotides identify conserved and variable regions of pspA and pspA-like sequences of Streptococcus pneumoniae.
Oligonucleotides identify conserved and variable regions of pspA and pspA-like sequences of Streptococcus pneumoniae.
复制标题
寡核苷酸可识别肺炎链球菌 pspA 和 pspA 样序列的保守区和可变区。
DOI：
10.1016/s0378-1119(96)00823-2
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发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
McDaniel,LS
中科院分区：
文献类型：
--
作者：
Swiatlo,E;Brooks-Walter,A;Briles,DE;McDaniel,LS
肺炎球菌表面蛋白A（PspA）是肺炎链球菌的免疫原性表面蛋白。的PspA。肺炎杆菌菌株Rx 1是一种65 kDa蛋白，由288个氨基酸的α-螺旋N-末端，随后是82个氨基酸的脯氨酸富集区，10个重复序列，每个重复序列20个氨基酸，和17个氨基酸的C-末端组成。pspA基因的3′端相对保守，5′端变异较大。此外，几乎所有的肺炎球菌菌株都含有至少一个与pspA具有序列同源性的其他基因座。在这项研究中，来自Rx 1的pspA的DNA序列的寡核苷酸被用来作为杂交探针和作为引物的聚合酶链反应（PCR），以调查内的结构域的pspA和pspA样序列的遗传变异，从18株代表12个胶囊和9 PspA血清型。编码前导肽、富含脯氨酸的区域和重复区域的序列在pspA和pspA样序列之间高度保守。不同菌株的pspA和pspA样序列之间的α-螺旋编码结构域是高度多样的。© 1997 Elsevier Science B. V.保留所有权利。
Pneumococcal surface protein A (PspA) is an immunogenic surface protein of Streptococcus pneumoniae. PspA of S. pneumoniae strain Rx1 is a 65-kDa protein composed of an α-helical N-terminus of 288 amino acids followed by an 82-amino-acid proline-rich region, 10 repeats of 20 amino acids each, and a 17-amino-acid C-terminus. It has been demonstrated that the 3′-half of pspA is relatively conserved among unrelated pneumococcal isolates and the 5′-half of the gene is highly variable. Additionally, nearly all pneumococcal strains contain at least one other locus with sequence homology to pspA. In this study oligonucleotides derived from the DNA sequence of pspA of Rx1 were used both as hybridization probes and as primers in the polymerase chain reaction (PCR) to investigate genetic variation within domains of pspA and in the pspA-like sequences from 18 strains representing 12 capsule and 9 PspA serotypes. Sequences encoding the leader peptide, the proline-rich region, and the repeat region are highly conserved among pspA and pspA-like sequences. The α-helical coding domain is highly diverse among pspA and pspA-like sequences of different strains. © 1997 Elsevier Science B.V. All rights reserved.