Tobacco exposure associated with oral microbiota oxygen utilization in the New York City Health and Nutrition Examination Study
Tobacco exposure associated with oral microbiota oxygen utilization in the New York City Health and Nutrition Examination Study
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DOI：
10.1016/j.annepidem.2019.03.005
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发表时间：
2019-06-01
影响因子：
5.6
通讯作者：
Waldron, Levi
中科院分区：
文献类型：
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作者：
Beghini, Francesco;Renson, Audrey;Waldron, Levi
目的：烟草暴露对口腔微生物组的影响尚未建立。方法：我们对16 S核糖体RNA基因V4可变区进行扩增子测序，以估计259个口腔冲洗液样本中的细菌群落特征，这些样本是根据2013-2014年纽约市健康和营养检查研究中自我报告的吸烟和血清可替宁水平选择的。我们通过初次和二手烟草暴露确定了差异丰富的操作分类单位（OTU），并使用“微生物集富集分析”来评估微生物氧利用的变化。吸烟与需氧OTU的消耗有关（富集分数检验统计量ES =-0.75，P = .002），与从不吸烟者相比，当前吸烟者中富集了少数（29%）需氧OTU。通过血清可替宁水平测量，观察到当前吸烟者与二手暴露的非吸烟者的微生物群一致的变化。差异丰度的结果是类似的粗和调整analysis.Conclusions：结果支持烟草暴露和口腔微生物组的变化之间的合理联系，在人口水平上通过三条证据线：（1）与主要烟草烟雾暴露相关的微生物群氧利用的变化;（2）与当前吸烟相关的丰度倍数变化的一致性和非吸烟者二手烟暴露梯度的沿着变化;和（3）调整先验假设混杂因素后的一致性。(C)2019爱思唯尔公司All rights reserved.
Purpose: The effect of tobacco exposure on the oral microbiome has not been established.Methods: We performed amplicon sequencing of the 16S ribosomal RNA gene V4 variable region to estimate bacterial community characteristics in 259 oral rinse samples, selected based on self-reported smoking and serum cotinine levels, from the 2013-2014 New York City Health and Nutrition Examination Study. We identified differentially abundant operational taxonomic units (OTUs) by primary and secondhand tobacco exposure, and used "microbe set enrichment analysis" to assess shifts in microbial oxygen utilization.Results: Cigarette smoking was associated with depletion of aerobic OTUs (Enrichment Score test statistic ES = -0.75, P = .002) with a minority (29%) of aerobic OTUs enriched in current smokers compared with never smokers. Consistent shifts in the microbiota were observed for current cigarette smokers as for nonsmokers with secondhand exposure as measured by serum cotinine levels. Differential abundance findings were similar in crude and adjusted analyses.Conclusions: Results support a plausible link between tobacco exposure and shifts in the oral microbiome at the population level through three lines of evidence: (1) a shift in microbiota oxygen utilization associated with primary tobacco smoke exposure; (2) consistency of abundance fold changes associated with current smoking and shifts along the gradient of secondhand smoke exposure among nonsmokers; and (3) consistency after adjusting for a priori hypothesized confounders. (C) 2019 Elsevier Inc. All rights reserved.