Porphyromonas gingivalis Antigens and Interleukin-6 Stimulate the Production of Monocyte Chemoattractant Protein-1 via the Upregulation of Early Growth Response-1 Transcription in Human Coronary Artery Endothelial Cells
Porphyromonas gingivalis Antigens and Interleukin-6 Stimulate the Production of Monocyte Chemoattractant Protein-1 via the Upregulation of Early Growth Response-1 Transcription in Human Coronary Artery Endothelial Cells
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DOI：
10.1159/000265568
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发表时间：
2010-01-01
影响因子：
1.7
通讯作者：
Yamazaki, Kazuhisa
中科院分区：
文献类型：
--
作者：
Maekawa, Tomoki;Takahashi, Naoki;Yamazaki, Kazuhisa
背景资料：牙周炎患者血清IL-6和C-反应蛋白水平升高，据报道，患心血管疾病的风险显著增加。转录因子早期生长反应因子1（Egr-1）在动脉粥样硬化的发生和发展中起重要作用。然而，目前尚不清楚牙周感染是否影响Egr-1的表达和随后的内皮细胞表达单核细胞趋化蛋白（MCP）-1，白细胞化学吸引到血管的关键分子。研究方法：用牙龈卟啉单胞菌381或SU 63菌株的超声提取物或IL-6和可溶性IL-6受体（IL-6/sIL-6 R）的组合刺激人冠状动脉内皮细胞（HCAECs）。然后分析Egr-1和随后的MCP-1的表达。通过siRNA转染分析Egr-1对MCP-1表达的作用。结果：牙龈卟啉单胞菌抗原和IL-6/sIL-6 R刺激均能上调Egr-1的表达，IL-6/sIL-6 R的作用更强。Egr-1的表达增加与MCP-1的产生相一致，而siRNA下调Egr-1则抑制了这种作用。结论：这些结果清楚地表明，牙周感染有可能影响HCAEC，从而有助于随后的动脉粥样硬化的发展。版权所有（C）2009 S. Karger AG，巴塞尔
Background: Individuals with periodontitis have elevated serum levels of IL-6 and C-reactive protein and have been reported to have a significantly increased risk of developing cardiovascular disease. The transcription factor early growth response factor 1 (Egr-1) has been shown to play an important role in the development and progression of atherosclerosis. However, it is not known whether periodontal infection affects the expression of Egr-1 and subsequent endothelial cells expression of monocyte chemoattractant protein (MCP)-1, a key molecule of leukocyte chemoattraction into vessels. Methods: Human coronary artery endothelial cells (HCAECs) were stimulated with either sonicated extracts from Porphyromonas gingivalis strains 381 or SU63, or a combination of IL-6 and soluble IL-6 receptor (IL-6/sIL-6R). The expression of Egr-1, and subsequently MCP-1, was then analyzed. The role of Egr-1 on MCP-1 expression was analyzed by siRNA transfection. Results: Both P. gingivalis anti-gens and IL-6/sIL-6R stimulations upregulated the expression of Egr-1, with a more robust effect by IL-6/sIL-6R. Increased expression of Egr-1 coincided with MCP-1 production, and Egr-1 downregulation by siRNA suppressed this effect. Conclusion: These results clearly suggest that periodontal infection has the potential to affect HCAECs and hence contribute to the development of subsequent atherosclerosis. Copyright (C) 2009 S. Karger AG, Basel