Serotonin in the developing stomatogastric system of the lobster, Homarus americanus.
Serotonin in the developing stomatogastric system of the lobster, Homarus americanus.
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龙虾（Homarus americanus）发育中的口胃系统中的血清素。
DOI：
10.1002/neu.10136
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Marder,Eve
中科院分区：
文献类型：
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作者：
Richards,KathrynS;Simon,DavidJ;Pulver,StefanR;Beltz,BarbaraS;Marder,Eve
我们研究了美洲白鹤龙虾口胃神经系统的血清素调节的发展。虽然口胃神经节 (STG) 在胚胎发育早期就存在，但直到第二个幼虫阶段，STG 中才可见血清素免疫反应性。然而，STG 与外源血清素的孵育表明，血清素转运蛋白存在于胚胎和早期幼虫阶段。帕罗西汀和 0% Na+ 盐水阻断血清素的摄取。胚胎 STG 中血清素转运蛋白的存在表明，激素释放的血清素可以被 STG 吸收，并可能作为“借来的递质”释放。与潜在的激素作用一致，通过中胚发育，在心包器官（一种主要的神经分泌结构）中发现了血清素。血清素降低了胚胎和幼虫 STG 产生的节律性运动模式的频率。胚胎和第一幼虫阶段 (LI) 中的侧幽门 (LP) 神经元诱发的兴奋性连接电位 (EJP) 比成人中的更大、更慢且变化更大。成人 LP 神经元诱发的 EJP 幅度在血清素中增加两倍以上，但在胚胎和 LI 制剂中，这种影响可以忽略不计。在胚胎和 LI 制剂中，5-羟色胺增加了肌纤维动作电位的发生并改变了 EJP 波形。这些数据表明，血清素受体存在于发育早期的口胃神经系统中，并表明血清素的作用从发育早期调节肌纤维兴奋性转变为成人中神经诱发 EJP 的增强。 © 2002 Wiley periodicals, Inc. J Neurobiol 54: 380–392, 2003
We studied the development of the serotonergic modulation of the stomatogastric nervous system of the lobster,Homarus americanus. Although the stomatogastric ganglion (STG) is present early in embryonic development, serotonin immunoreactivity is not visible in the STG until the second larval stage. However, incubation of the STG with exogenous serotonin showed that a serotonin transporter is present in embryonic and early larval stages. Serotonin uptake was blocked by paroxetine and 0% Na+saline. The presence of a serotonin transporter in the embryonic STG suggests that hormonally liberated serotonin could be taken up by the STG, and potentially released as a “borrowed transmitter”. Consistent with a potential hormonal role, serotonin is found in the pericardial organs, a major neurosecretory structure, by midembryonic development. The rhythmic motor patterns produced by embryonic and larval STGs were decreased in frequency by serotonin. Lateral Pyloric (LP) neuron‐evoked excitatory junctional potentials (EJPs) in the embryos and the first larval stage (LI) were larger, slower, and more variable than those in the adult. The amplitude of adult LP neuron‐evoked EJPs was increased more than twofold in serotonin, but in embryos and LI preparations this effect was negligible. In embryos and LI preparations, serotonin increased the occurrence of muscle fiber action potentials and altered the EJP wave‐form. These data demonstrate that serotonin receptors are present in the stomatogastric nervous system early in development, and suggest that the role of serotonin changes from modulation of muscle fiber excitability early in development to enhancement of neurally evoked EJPs in the adult. © 2002 Wiley Periodicals, Inc. J Neurobiol 54: 380–392, 2003