Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii
Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii
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DOI：
10.1093/jxb/erh113
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发表时间：
2004-05-01
影响因子：
6.9
通讯作者：
Volokita, M
中科院分区：
文献类型：
--
作者：
Mittova, V;Guy, M;Volokita, M
研究了盐胁迫对栽培番茄Lycopersicon esculentum（Lem）及其野生近缘种L. pennellii（Lpa）进行了研究。盐胁迫诱导的氧化应激在莱姆线粒体，所示的脂质过氧化和H2O2水平的增加。这些变化与超氧化物歧化酶（SOD）和愈创木酚过氧化物酶（POD）活性以及抗坏血酸（ASC）和谷胱甘肽（GSH）含量的降低有关。与此相反，在线粒体中的盐处理Lpa植物H2O2和脂质过氧化水平下降，而ASC和GSH的水平和SOD的活性，抗坏血酸过氧化物酶（APX）的几种异构体，和POD的增加。类似的线粒体，过氧化物酶体从盐处理的LPA植物的根分离也表现出降低水平的脂质过氧化和H2O2和增加SOD，抗坏血酸过氧化物酶（APX），和过氧化氢酶（CAT）的活动。尽管盐胁迫降低了Lem过氧化物酶体中抗氧化酶的活性，但这些细胞器中的氧化应激并不明显。
The effect of salinity on the antioxidative system of root mitochondria and peroxisomes of a cultivated tomato Lycopersicon esculentum (Lem) and its wild salt-tolerant related species L. pennellii (Lpa) was studied. Salt stress induced oxidative stress in Lem mitochondria, as indicated by the increased levels of lipid peroxidation and H2O2. These changes were associated with decreased activities of superoxide dismutase (SOD) and guaiacol peroxidases (POD) and contents of ascorbate (ASC) and glutathione (GSH). By contrast, in mitochondria of salt-treated Lpa plants both H2O2 and lipid peroxidation levels decreased while the levels of ASC and GSH and activities of SOD, several isoforms of ascorbate peroxidase (APX), and POD increased. Similarly to mitochondria, peroxisomes isolated from roots of salt-treated Lpa plants exhibited also decreased levels of lipid peroxidation and H2O2 and increased SOD, ascorbate peroxidase (APX), and catalase (CAT) activities. In spite of the fact that salt stress decreased activities of antioxidant enzymes in Lem peroxisome, oxidative stress was not evident in these organelles.