Alternative mRNA Editing in Trypanosomes Is Extensive and May Contribute to Mitochondrial Protein Diversity
Alternative mRNA Editing in Trypanosomes Is Extensive and May Contribute to Mitochondrial Protein Diversity
复制标题
DOI：
10.1371/journal.pone.0001566
复制
发表时间：
2008-02-13
期刊：
影响因子：
3.7
通讯作者：
Hajduk, Stephen L.
中科院分区：
文献类型：
--
作者：
Ochsenreiter, Torsten;Cipriano, Michael;Hajduk, Stephen L.
锥虫线粒体mRNA的编辑产生线粒体功能所必需的转录物，包括电子传递和氧化磷酸化。前体mRNA通常通过小向导RNA（gRNA）指导的特定尿苷插入或缺失进行广泛编辑。最近，研究表明，细胞色素c氧化酶亚基III（COXIII）mRNA可以进行选择性编辑，编码一种新型线粒体膜蛋白，该蛋白由功能未知的独特亲水性N末端序列和COXIII的C末端疏水性片段组成。为了扩展布氏锥虫中替代编辑的分析，我们构建了具有超过1100个全长线粒体cDNA和超过1200个gRNA基因的序列的文库。使用这些数据，我们表明COXIII，ATP酶亚基6（A6）和NADH脱氢酶亚基7，8和9（ND 7，8，9）mRNA的交替编辑可以产生新的开放阅读框架（ORF）。还鉴定了几种可能负责这些mRNA的替代编辑的gRNA。这些发现表明，线粒体mRNA的选择性编辑在T。布氏杆菌，并扩大了这些生物体中线粒体蛋白的多样性。
The editing of trypanosome mitochondrial mRNAs produces transcripts necessary for mitochondrial functions including electron transport and oxidative phosphorylation. Precursor-mRNAs are often extensively edited by specific uridine insertion or deletion that is directed by small guide RNAs (gRNAs). Recently, it has been shown that cytochrome c oxidase subunit III (COXIII) mRNAs can be alternatively edited to encode a novel mitochondrial membrane protein composed of a unique hydrophilic N-terminal sequence of unknown function and the C-terminal hydrophobic segment of COXIII. To extend the analysis of alternative editing in Trypanosoma brucei we have constructed libraries with over 1100 full-length mitochondrial cDNAs and the sequences of over 1200 gRNA genes. Using this data, we show that alternative editing of COXIII, ATPase subunit 6 (A6), and NADH dehydrogenase subunits 7, 8 and 9 (ND7, 8, 9) mRNAs can produce novel open reading frames (ORFs). Several gRNAs potentially responsible for the alternative editing of these mRNAs were also identified. These findings show that alternative editing of mitochondrial mRNAs is common in T. brucei and expands the diversity of mitochondrial proteins in these organisms.