Injection of ultrasmall samples and single molecules into tapered capillaries
Injection of ultrasmall samples and single molecules into tapered capillaries
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DOI：
10.1021/ac961226g
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发表时间：
1997-05-15
影响因子：
7.4
通讯作者：
Zare, RN
中科院分区：
文献类型：
--
作者：
Chiu, DT;Hsiao, A;Zare, RN
描述了一种新的注射方案，其中在10(-18)L和10(-15)L范围内的超小样品，甚至是单分子，被可控地引入锥形毛细管，以便进行电泳分离。为了使毛细管入口的尺寸与样品的尺寸相匹配，毛细管尖端的内径从数百纳米到几微米不等。为了注入一个超小型样品，光学捕获被用来固定和操作样品，以便将其放置在毛细管入口内或旁边。一个可控的小吸力使样品进入毛细管。
A novel injection scheme is described in which ultrasmall samples in the attoliter (10(-18) L) and low femtoliter (10(-15) L) range, or even single molecules, are controllably introduced into a tapered capillary so that electrophoretic separation can be carried out. To match the dimensions of the capillary inlet with that of the sample, capillary tips are tapered to an inside diameter ranging from hundreds of nanometers to a few micrometers. To inject an ultrasmall sample, optical trapping is used to immobilize and manipulate the sample in order to place it inside or next to the capillary inlet. A small controlled suction results in the loading of the sample into the capillary.