Hyaluronic Acid/Poly(lactic-co-glycolic acid) Core/Shell Fiber Meshes Loaded with Epigallocatechin-3-O-Gallate as Skin Tissue Engineering Scaffolds
Hyaluronic Acid/Poly(lactic-co-glycolic acid) Core/Shell Fiber Meshes Loaded with Epigallocatechin-3-O-Gallate as Skin Tissue Engineering Scaffolds
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DOI：
10.1166/jnn.2014.9922
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发表时间：
2014-11-01
影响因子：
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通讯作者：
Han, Dong-Wook
中科院分区：
文献类型：
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作者：
Lee, Eun Ji;Lee, Jong Ho;Han, Dong-Wook
本研究采用同轴静电纺丝法制备了透明质酸（HA）/聚乳酸-羟基乙酸共聚物（PLGA）核/壳纤维网（HA/PLGA-E）负载表没食子儿茶素-3-O-没食子酸酯（EGCG）用于皮肤再生组织工程支架。采用扫描电镜、拉曼光谱、接触角、表没食子儿茶素没食子酸酯（EGCG）释放曲线和体外降解等方法对HA/PLGA-E核/壳纤维网的理化性质进行表征。HA/PLGA-E补片的生物力学性能也通过拉伸强度测试进行了研究。SEM图像显示HA/PLGA-E纤维网具有三维互连的孔结构，平均纤维直径约为1270 nm。拉曼光谱显示，EGCG均匀分散在PLGA壳层的网片中。HA/PLGA-E网片通过控制扩散和PLGA降解在4周内显示出持续的EGCG释放模式。EGCG负载量对HA/PLGA网片的拉伸强度和弹性模量没有不利影响，但增加了其亲水性和表面能。HA/PLGA-E补片上的人真皮成纤维细胞的附着明显增加，并且在培养期间稳定保持其增殖。这些结果表明，HA/PLGA-E核/壳纤维网可以潜在地用作支持皮肤再生的支架。
In this study, hyaluronic acid (HA)/poly(lactic-co-glycolic acid, PLGA) core/shell fiber meshes loaded with epigallocatechin-3-O-gallate (EGCG) (HA/PLGA-E) for application to tissue engineering scaffolds for skin regeneration were prepared via coaxial electrospinning. Physicochemical properties of HA/PLGA-E core/shell fiber meshes were characterized by SEM, Raman spectroscopy, contact angle, EGCG release profiling and in vitro degradation. Biomechanical properties of HA/PLGA-E meshes were also investigated by a tensile strength test. SEM images showed that HA/PLGA-E fiber meshes had a three-dimensional interconnected pore structure with an average fiber diameter of about 1270 nm. Raman spectra revealed that EGCG was uniformly dispersed in the PLGA shell of meshes. HA/PLGA-E meshes showed sustained EGCG release patterns by controlled diffusion and PLGA degradation over 4 weeks. EGCG loading did not adversely affect the tensile strength and elastic modulus of HA/PLGA meshes, while increased their hydrophilicity and surface energy. Attachment of human dermal fibroblasts on HA/PLGA-E meshes was appreciably increased and their proliferation was steadily retained during the culture period. These results suggest that HA/PLGA-E core/shell fiber meshes can be potentially used as scaffolds supporting skin regeneration.