Differences in Oral Structure and Tissue Interactions during Mouse vs. Human Palatogenesis: Implications for the Translation of Findings from Mice.
Differences in Oral Structure and Tissue Interactions during Mouse vs. Human Palatogenesis: Implications for the Translation of Findings from Mice.
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DOI：
10.3389/fphys.2017.00154
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发表时间：
2017
影响因子：
4
通讯作者：
Cox TC
中科院分区：
文献类型：
--
作者：
Yu K;Deng M;Naluai-Cecchini T;Glass IA;Cox TC
继发性腭裂是人类最常见的出生缺陷之一，是由于胚胎发育过程中腭架未能融合所致。腭裂的发生在哺乳动物中传统上被认为是一个高度保守的发育过程。然而，人类的腭裂表型比小鼠的表型变化要大得多，小鼠是研究腭裂发育和发病机制的最常见的动物模型。在这项研究中，我们利用宏观观察、组织学和3D成像技术来直接比较小鼠胚胎和人类胚胎在腭部闭合过程中的腭部形态和口腔-鼻腔。我们发现，由于口腔-鼻腔的结构差异，老鼠和人类的味觉显示出不同的形态。我们进一步表明，在小鼠和人类的上颌闭合过程中，腭架与硬腭区的初级腭部和鼻中隔以及软腭区的咽壁之间的相互作用不同。了解这些形态差异对于更好地翻译人类唇腭裂小鼠模型的发现是重要的，因此，最终应该加强我们对人类腭裂形态发生和人类唇腭裂发病机制的理解。
Clefting of the secondary palate is one of the most common human birth defects and results from failure of the palatal shelves to fuse during embryonic development. Palatogenesis is traditionally considered to be a highly conserved developmental process among mammalian species. However, cleft palate phenotypes in humans are considerably more variable than those seen in mice, the most common animal model for studying palatal development and pathogenesis of cleft palate. In this investigation, we utilized macroscopic observations, histology and 3D imaging techniques to directly compare palate morphology and the oral-nasal cavity during palate closure in mouse embryos and human conceptuses. We showed that mouse and human palates display distinct morphologies attributable to the structural differences of the oral-nasal cavity. We further showed that the palatal shelves interact differently with the primary palate and nasal septum in the hard palate region and with pharyngeal walls in the soft palate region during palate closure in mice and humans. Knowledge of these morphological differences is important for improved translation of findings in mouse models of human cleft lip/palate and, as such, should ultimately enhance our understanding of human palatal morphogenesis and the pathogenesis of cleft lip/palate in humans.