Light/dark phase-dependent spontaneous activity is maintained in dopamine-deficient mice.
Light/dark phase-dependent spontaneous activity is maintained in dopamine-deficient mice.
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DOI：
10.1186/s13041-017-0329-4
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发表时间：
2017-10-16
期刊：
影响因子：
3.6
通讯作者：
Ikeda K
中科院分区：
文献类型：
--
作者：
Fujita M;Hagino Y;Takeda T;Kasai S;Tanaka M;Takamatsu Y;Kobayashi K;Ikeda K
多巴胺对运动控制很重要，并参与昼夜节律的调节。我们以前发现，多巴胺缺乏（DD）小鼠在一个新的环境中变得多动72小时后，最后一次注射L-3，4-二羟基苯丙氨酸（L-DOPA）时，多巴胺几乎完全耗尽。DD小鼠最初在它们的饲养笼中没有表现出活动过度，但在最后一次注射L-DOPA后几小时，动物表现出活动过度。在新的环境和在家里的笼子里的运动活动的调节可能是不同的。先前的研究报道，DD小鼠在最后一次注射L-DOPA后约24小时再次变得活跃。一种推测是，尽管多巴胺缺乏，但依赖于光/暗相位的自发活动可能会维持。本研究调查了在最后一次注射L-DOPA后24-43 h和72-91 h DD小鼠中是否维持自发的家笼活动。在最后一次注射L-DOPA后24和72小时，DD小鼠在光/暗周期的光相期间的自发活动几乎完全受到抑制。在黑暗阶段开始后，DD小鼠在最后一次注射L-DOPA后24和72小时变得活跃。在最后一次注射L-DOPA后24小时，DD小鼠表现出与野生型小鼠相似的自发活动量。尽管DD小鼠在最后一次注射L-DOPA后72小时活动减少，但它们保持暗相刺激的运动激活。尽管DD小鼠的多巴胺水平较低，但它们表现出与野生型小鼠相似的进食行为。虽然梳理和饲养行为显着下降，DD小鼠保留了他们的能力，进行这些活动。氟哌啶醇治疗显著抑制野生型小鼠的所有这些行为，但在DD小鼠中没有。这些结果表明，DD小鼠保持某些方面的光/暗相依赖的自发活动，尽管多巴胺耗竭，这表明补偿多巴胺独立的机制可能发挥作用的DD小鼠表型。
Dopamine is important for motor control and involved in the regulation of circadian rhythm. We previously found that dopamine-deficient (DD) mice became hyperactive in a novel environment 72 h after the last injection of L-3,4-dihydroxyphenylalanine (L-DOPA) when dopamine was almost completely depleted. DD mice did not initially exhibit hyperactivity in their home cages, but the animals exhibited hyperactivity several hours after the last L-DOPA injection. The regulation of motor activity in a novel environment and in home cages may be different. A previous study reported that DD mice became active again approximately 24 h after the last L-DOPA injection. One speculation was that light/dark phase-dependent spontaneous activity might be maintained despite dopamine deficiency. The present study investigated whether spontaneous home cage activity is maintained in DD mice 24–43 h and 72–91 h after the last L-DOPA injection. Spontaneous activity was almost completely suppressed during the light phase of the light/dark cycle in DD mice 24 and 72 h after the last L-DOPA injection. After the dark phase began, DD mice became active 24 and 72 h after the last L-DOPA injection. DD mice exhibited a similar amount of locomotor activity as wildtype mice 24 h after the last L-DOPA injection. Although DD mice presented a decrease in activity 72 h after the last L-DOPA injection, they maintained dark phase-stimulated locomotor activation. Despite low levels of dopamine in DD mice, they exhibited feeding behavior that was similar to wildtype mice. Although grooming and rearing behavior significantly decreased, DD mice retained their ability to perform these activities. Haloperidol treatment significantly suppressed all of these behaviors in wildtype mice but not in DD mice. These results indicate that DD mice maintain some aspects of light/dark phase-dependent spontaneous activity despite dopamine depletion, suggesting that compensatory dopamine-independent mechanisms might play a role in the DD mouse phenotype.
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影响因子：
5.3
作者：
Korshunov KS;Blakemore LJ;Trombley PQ
通讯作者：
Trombley PQ
DOI：
10.1073/pnas.0603081103
发表时间：
2006-06-06
影响因子：
11.1
作者：
Hnasko, Thomas S.;Perez, Francisco A.;Palmiter, Richard D.
通讯作者：
Palmiter, Richard D.
影响因子：
8.6
作者：
Soykan, I;Sarosiek, I;McCallum, RW
通讯作者：
McCallum, RW
影响因子：
11
作者：
Bello, E. P.;Casas-Cordero, R.;Rubinstein, M.
通讯作者：
Rubinstein, M.
影响因子：
3.6
作者：
CHANDLER, CJ;STARR, BS;STARR, MS
通讯作者：
STARR, MS