Polyamine synthesis from arginine and proline in tissues of developing chickens
Polyamine synthesis from arginine and proline in tissues of developing chickens
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DOI：
10.1007/s00726-021-03084-7
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发表时间：
2021-10-06
期刊：
影响因子：
3.5
通讯作者：
Wu, Guoyao
中科院分区：
文献类型：
--
作者：
Furukawa, Kyohei;He, Wenliang;Wu, Guoyao
多胺（腐胺、亚精胺和精胺）主要在哺乳动物中通过鸟氨酸脱羧酶（ODC）由鸟氨酸合成。虽然鸟类组织含有ODC活性，但对用于多胺合成的鸟氨酸的细胞内来源知之甚少。本研究验证了脯氨酸氧化酶和丝氨酸蛋白酶参与鸡体内多胺合成的假说。测定了0、7、14和21日龄肉仔鸡肾脏、空肠、腿肌和肝脏组织中过氧化物酶、脯氨酸氧化酶（POX）、鸟氨酸氨基转移酶（OAT）和鸟氨酸脱羧酶（ODC）的活性。还使用肾切片来确定从[U-C-14]精氨酸和[U-C-14]脯氨酸合成C-14-多胺。此外，分析这些组织和血浆中的多胺。结果表明，所有组织都含有OAT（线粒体）和ODC（胞质）活性，但过氧化物酶和过氧化物酶仅在鸡肾线粒体中检测到。肾脏POX和过氧化物酶活性在7日龄时比新孵化的鸟类更大，并在第14天下降。与14日龄相比，21日龄时肾过氧化物酶活性更高，但在同一时期肾过氧化物酶活性没有变化。与第0天相比，第7天肾脏和血浆中的多胺浓度较高，随后在第14天和第21天降低。肾脏切片容易将精氨酸和脯氨酸转化为多胺，峰值速率在第7天。鸡血浆中腐胺、亚精胺和精胺的浓度约为哺乳动物的20至100倍。我们的研究结果表明，多胺合成精氨酸和脯氨酸在鸟类肾脏。与哺乳动物不同，肾脏释放的多胺可能是鸡血液和其他肾外组织中多胺的主要来源。
Polyamines (putrescine, spermidine, and spermine) are synthesized primarily from ornithine via ornithine decarboxylase (ODC) in mammals. Although avian tissues contain ODC activity, little is known about intracellular sources of ornithine for their polyamine synthesis. This study tested the hypothesis that arginase and proline oxidase contribute to polyamine synthesis in chickens. Kidney, jejunum, leg muscle, and liver from 0-, 7-, 14- and 21-day-old broiler chickens were assayed for the activities of arginase, proline oxidase (POX), ornithine aminotransferase (OAT), and ornithine decarboxylase (ODC). Kidney slices were also used to determine C-14-polyamine synthesis from [U-C-14]arginine and [U-C-14]proline. Furthermore, these tissues and plasma were analyzed for polyamines. Results indicate that all tissues contained OAT (mitochondrial) and ODC (cytosolic) activities, but arginase and POX activities were only detected in the mitochondria of chicken kidneys. Renal POX and arginase activities were greater at 7 days of age compared to newly hatched birds, and declined by Day 14. Renal arginase activity was greater at 21 days compared to 14 days of age, but there was no change in renal POX activity during that same period. Concentrations of polyamines in the kidneys and plasma were greater on Day 7 compared to Day 0 and decreased thereafter on Days 14 and 21. Kidney slices readily converted arginine and proline into polyamines, with peak rates being on Day 7. Concentrations of putrescine, spermidine and spermine in the plasma of chickens were about 20- to 100-fold greater than those in mammals. Our results indicate that polyamines are synthesized from arginine and proline in avian kidneys. Unlike mammals, polyamines released from the kidneys are likely the major source of polyamines in the blood and other extra-renal tissues in chickens.