Mating system of Brazilian Oryza glumaepatula populations studied with microsatellite markers.
Mating system of Brazilian Oryza glumaepatula populations studied with microsatellite markers.
复制标题
用微卫星标记研究巴西稻种群的交配系统。
DOI：
10.1093/aob/mcl248
复制
发表时间：
2006
期刊：
影响因子：
4.2
通讯作者：
E. Veasey
中科院分区：
文献类型：
--
作者：
M. G. Karasawa;R. Vencovsky;Cynthia M Silva;M. Zucchi;G. C. Oliveira;E. Veasey
背景与目的
了解自然种群的繁殖系统对于实施原地和迁地管理和养护做法十分重要。利用微卫星标记对来自巴西的3个颖壳稻群体进行了研究，以确定该物种的育种系统和遗传结构参数。
方法
每个群体代表10个家庭，每个家庭10个个体进行了研究，使用8个微卫星引物。Rio Xingu种群（XI）的家系从温室获得，而Rio Solimoes（SO）和Rio Paraguay（PG）的家系从野外收集。用银染法观察在非变性聚丙烯酰胺凝胶上电泳的扩增产物。采用混合交配模型（MLTR软件）分析了3个群体的交配系统参数，采用FSTAT软件对3个群体及其家系进行了遗传结构分析。
关键成果
XI、PG和SO群体的平均等位基因数分别为2.5、3.9和2.5。与它们的家庭相比，更高的值所观察到的杂合性和基因多样性估计的父母群体。在SO和PG群体中，基于R（ST）的细分和近交（F（IS））具有相似的效应，而在家系中，近交是主要效应。3个群体的多位点异交率在0.011~0.223之间，表明O. glumaepatula种群。对于物种（同时考虑SO和PG种群），观察到中间值（tm = 0.116）。双亲近交系数在0.008~0.123之间，对这些群体的自交率有贡献。超过50%的异交发生在相关个体之间。
结论
结果表明，在交配系统上，O. glumaepatula种群，与保护措施的后果。该物种的交配系统被归类为混合与优势的自花受精。
BACKGROUND AND AIMS
A knowledge of natural populations' breeding systems is important in order to implement in situ and ex situ management and conservation practices. Using microsatellite markers, three Oryza glumaepatula populations from Brazil were studied to determine the breeding system and genetic structure parameters of this species.
METHODS
Each population represented by ten families with ten individuals per family was studied using eight microsatellite primers. Families of the Rio Xingu population (XI) were obtained from the greenhouse, whereas families from Rio Solimoes (SO) and Rio Paraguay (PG) were collected from the wild. Amplified products electrophoresed on non-denaturing polyacrylamide gels were visualized with a silver staining procedure. The mating system parameters were analysed based on the mixed mating model (software MLTR) while genetic structure analyses of the three populations and their families were performed using the FSTAT software.
KEY RESULTS
The mean numbers of alleles per loci were 2.5, 3.9 and 2.5, respectively for the XI, PG and SO populations. Compared with their families, higher values for the observed heterozygosity and gene diversity were estimated for the parental populations. The subdivision (based on R(ST)) and inbreeding (F(IS)) in the SO and PG populations had similar effects, while inbreeding was the main effect in the families. Multilocus outcrossing rates varied from 0.011 to 0.223 in the three populations, indicating divergence in the outcrossing rates among O. glumaepatula populations. For the species (considering SO and PG populations together) an intermediate value was observed (tm = 0.116). Biparental inbreeding varied from 0.008 to 0.123, contributing to the selfing rate in these populations. More than 50 % of the outcrossing occurred between related individuals.
CONCLUSIONS
The results indicated divergence in the mating system among O. glumaepatula populations, with consequences for conservation practices. The mating system of this species was classified as mixed with a predominance of self-fertilization.