TUBULAR CRYSTALS OF ACETYLCHOLINE-RECEPTOR
TUBULAR CRYSTALS OF ACETYLCHOLINE-RECEPTOR
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DOI：
10.1083/jcb.99.4.1202
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发表时间：
1984-01-01
影响因子：
7.8
通讯作者：
UNWIN, PNT
中科院分区：
文献类型：
--
作者：
BRISSON, A;UNWIN, PNT
从鱼雷富含受体的囊泡悬浮液中获得了有序的乙酰胆碱受体管状晶体。它们是由排列在表面晶格上的一对相反取向的分子组成的，具有平面群p2的对称性(平均晶胞尺寸：a=90，b=162，…)。=117度)该晶格中的受体在其周长周围质量分布不对称，但呈规则的五角形；因此，其5个跨膜亚基似乎具有不同的长度，但截面积大致相等。在配对分子的囊泡表面，管子以横向聚集的方式生长。胶带和胶管都对二硫代还原剂二硫苏糖醇的分散很敏感。这一观察和其他证据表明，管子中的基本配对相互作用可能是生理二聚体的相互作用，涉及β-亚基之间的接触。
Well-ordered tubular crystals of acetylcholine receptor were obtained from suspensions of Torpedo marmorata receptor-rich vesicles. They are composed of pairs of oppositely oriented molecules arranged on the surface lattice with the symmetry of the plane group p2 (average unit cell dimensions: a = 90 .ANG., b = 162 .ANG., .gamma. = 117.degree.). The receptor in this lattice has an asymmetric distribution of mass around its perimeter, yet a regular pentagonal shape; thus its 5 transmembrane subunits appear to have different lengths, but approximately equal cross sections. The tubes grow by lateral aggregation on the vesicle surface of ribbons of the paired molecules. Both ribbons and tubes were sensitive to dispersal by the disulfide reductant, dithiothreitol. This observation and other evidence suggest that the basic pairing interaction in the tubes may be that of the physiological dimer, involving contact between .delta.-subunits.