A Metabolic Intravascular Platform to Study FDG Uptake in Vascular Injury.
A Metabolic Intravascular Platform to Study FDG Uptake in Vascular Injury.
复制标题
用于研究血管损伤中 FDG 摄取的代谢血管内平台。
DOI：
10.1007/s13239-020-00457-z
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发表时间：
2020
影响因子：
1.8
通讯作者：
Rodriguez-Porcel,M
中科院分区：
文献类型：
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作者：
Franchi,F;Olthoff,M;Krier,J;Noble,C;Al-Hijji,M;Ramaswamy,V;Witt,T;Burke,M;Benscoter,M;Lerman,A;Sandhu,GS;Rodriguez-Porcel,M
目的代谢改变是许多病理生理条件的基础，对它们的理解对于开发新的治疗方法至关重要。虽然体内代谢变化的评估在历史上一直具有挑战性，但分子成像的最新发展使我们能够直接在活体中研究新的代谢研究概念，使我们更接近患者。然而，在许多情况下，有必要的传感器，是在接近的器官调查，例如研究血管metabolis.MethodsIn这项研究中，我们开发和验证了代谢检测平台直接在活体受试者的炎症条件下。由一个放大光纤收集的信号使用光电倍增管放大，并由内部可调分析平台解码。在体内试验中，我们基于巨噬细胞的代谢特征进行实验，巨噬细胞与炎症密切相关，并渴望葡萄糖及其类似物18F-FDG（18F-FDG）。传感器在新西兰兔，其中炎症是由一个）高胆固醇（HC）饮食16周或B）血管球囊内皮剥脱，然后HC diet.ResultsThere是没有差异的重量，血流动力学，血压，或心率组之间。代谢传感器检测到血管炎症（炎症：0.60 ± 0.03 Au vs.对照：0.48 ± 0.03 Au，p= 0.01），即使血管内超声未检测到显著炎症/动脉粥样硬化，也强调了系统的高灵敏度。这些发现证实了巨噬细胞的存在离体主动脉tissue staining.ConclusionIn这项研究中，我们验证了一个可调的非常敏感的代谢传感器平台，可用于检测血管代谢，如炎症。该传感器不仅可用于检测巨噬细胞活性，而且与替代探针一起，它可以允许检测其他病理生理过程。
PurposeMetabolic alterations underlie many pathophysiological conditions, and their understanding is critical for the development of novel therapies. Although the assessment of metabolic changesin vivohas been historically challenging, recent developments in molecular imaging have allowed us to study novel metabolic research concepts directly in the living subject, bringing us closer to patients. However, in many instances, there is need for sensors that are in close proximity to the organ under investigation, for example to study vascular metabolism.MethodsIn this study, we developed and validated a metabolic detection platform directly in the living subject under an inflammatory condition. The signal collected by a scintillating fiber is amplified using a photomultiplier tube and decodified by an in-house tunable analysis platform. Forin vivotesting, we based our experiments on the metabolic characteristics of macrophages, cells closely linked to inflammation and avid for glucose and its analog18F-fluorodeoxyglucose (18F-FDG). The sensor was validated in New Zealand rabbits, in which inflammation was induced by either a) high cholesterol (HC) diet for 16 weeks or b) vascular balloon endothelial denudation followed by HC diet.ResultsThere was no difference in weight, hemodynamics, blood pressure, or heart rate between the groups. Vascular inflammation was detected by the metabolic sensor (Inflammation: 0.60 ± 0.03 AU vs. control: 0.48 ± 0.03 AU,p= 0.01), even though no significant inflammation/atherosclerosis was detected by intravascular ultrasound, underscoring the high sensitivity of the system. These findings were confirmed by the presence of macrophages on ex vivo aortic tissue staining.ConclusionIn this study, we validated a tunable very sensitive metabolic sensor platform that can be used for the detection of vascular metabolism, such as inflammation. This sensor can be used not only for the detection of macrophage activity but, with alternative probes, it could allow the detection of other pathophysiological processes.