Serotyping and Genetic Characterization of Hand, Foot, and Mouth Disease (HFMD)-Associated Enteroviruses of No-EV71 and Non-CVA16 Circulating in Fujian, China, 2011-2015.
Serotyping and Genetic Characterization of Hand, Foot, and Mouth Disease (HFMD)-Associated Enteroviruses of No-EV71 and Non-CVA16 Circulating in Fujian, China, 2011-2015.
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DOI：
10.12659/msm.901364
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发表时间：
2017-05-25
期刊：
影响因子：
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通讯作者：
Yan Y
中科院分区：
文献类型：
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作者：
Weng Y;Chen W;He W;Huang M;Zhu Y;Yan Y
手足口病(HFMD)是一种常见的婴幼儿传染病，由多种血清型的人类肠道病毒(EV)引起，属于微小病毒科肠病毒属。根据哨点监测，近年来手足口病患者感染EV71和CVA 16以外的EV变得越来越常见，这给手足口病控制提出了新的挑战。本研究旨在探索福建省东南部中国地区其他肠道病毒(非EV71型和非CVA16型)的血清型谱。我们调查了2011至2015年间经实时RT-PCR确诊的EVS感染手足口病患者的562份样本，并与其他EVS感染进行了比较。同时对肠道病毒进行核苷酸检测和血清学分型。扩增出完整的VP1基因，并进行了序列测定。对CVA6、CVA10、CVA4和CVA2进行了基于VP1的系统发育分析。从样品中鉴定出其他肠道病毒的22个血清型，分别属于4种人肠道病毒A-D。在22个血清型中，以CVA6(57.8%)和CVA10(21.0%)最常见，其次是CVA4(6.8%)和CVA2(2.7%)。其余18个血清型占样本总数的11.7%，均未超过2%。在47份(8.4%)重症手足口病患者标本中，检出10种血清型，其中CVA6型最多(20/47)，其次为CVA10(11/47)。VP1全序列比较表明，CVA6、CVA10、CVA4和CVA2的遗传相似性分别为96.7%、96.3%、94.4%和94.9%。基于VP1的系统发育分析表明，4种主要血清型存在不同的分支或亚支，提示福建其他肠道病毒的传播是复杂的。
Hand, foot, and mouth disease (HFMD) is a common contagious disease in infants; it is caused by multiple serotypes of human enterovirus (EV), which belongs to the enterovirus genus of the picornavirus family. According to sentinel surveillance, infection with EVs other than EV71 and CVA 16 have become increasingly common in recent years among HFMD patients, posing new challenges for HFMD control. This study aimed to explore the spectrum of serotypes in the other EVs (non-EV71 and non-CVA16) in Fujian province in southeastern China. We investigated 562 samples from EVs-infected HFMD patients with diagnosis confirmed by real-time RT-PCR with other EVs infection between 2011 and 2015. Nucleotide acid detection and the serotyping of the enteroviruses were also performed. The complete VP1 gene was amplified and sequenced. VP1-based phylogenetic analyses of CVA6, CVA10, CVA4, and CVA2 were also performed. Among the samples, 22 serotypes of the other EVs, which belong to 4 species of human enterovirus A–D, were identified. Of the 22 serotypes, CVA6 (57.8%) and CVA10 (21.0%) were most common, followed by CVA4 (6.8%) and CVA2 (2.7%). The other 18 serotypes accounted for 11.7% of samples, none of which exceeded 2%. Among 47 (8.4%) samples from patients with severe HFMD, 10 serotypes were identified and most samples belonged to CVA6 (20/47), followed by CVA10 (11/47). Entire VP1 comparison revealed that overall genetic identities were 96.7%, 96.3%, 94.4%, and 94.9% among strains within CVA6, CVA10, CVA4, and CVA2, respectively. VP1-based phylogenetic analysis for the 4 predominant serotypes indicated various clades or sub-clades, which suggests the complex transmissions of other enteroviruses in Fujian.