Imaging of metabolites using secondary ion mass spectrometry
Imaging of metabolites using secondary ion mass spectrometry
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DOI：
10.1007/s11306-012-0477-6
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发表时间：
2013-03-01
期刊：
影响因子：
3.6
通讯作者：
Lockyer, Nicholas P.
中科院分区：
文献类型：
--
作者：
Armitage, Emily G.;Kotze, Helen L.;Lockyer, Nicholas P.
本文对二次离子质谱仪成像技术进行了综述，并重点介绍了与代谢组学领域相关的一些当前和未来的应用领域。该方法得益于对分子物种的无标记分析，最大可达1500 Da，样品制备最少。该技术提供了当前质谱学成像方法中最高的空间分辨率，非常适合于在生物组织和细胞中进行2D和3D代谢物成像。
This article provides an overview of the technique of secondary ion mass spectrometry imaging and highlights some current and future areas of application relevant to the field of metabolomics. The approach benefits from label-free analysis of molecular species up to similar to 1500 Da with minimal sample preparation. Offering the highest spatial resolution of current mass spectrometry imaging methodologies, the technique is well-suited to metabolite imaging in both biological tissue and cells, in both 2D and 3D.