Using Xe as a heavy atom for phase determination of protein trichosanthin structure

Using Xe as a heavy atom for phase determination of protein trichosanthin structure
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以 Xe 作为重原子进行蛋白质天花粉蛋白结构的物相测定

DOI:
10.13538/j.1001-8042/nst.25.030502
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发表时间:
2014-06
影响因子:
2.8
通讯作者:
He Jian-Hua
He Jian-Hua
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Chen Ya-Xing;Li Min-Jun;Yu Feng;Liu Ke;Xu Chun-Yan;Sun Bo;Zhou Huan;Xu Qin;He Jian-Hua

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在1-3 Mpa的气压下,Xe可以结合到蛋白质疏水空腔中的离散位置,从而利用Xe作为重原子来测定蛋白质结晶学中的物相。利用单次反常散射衍射法,证明了
Under gas pressures of 1–3 MPa, xenon can be bound to discrete sites in hydrophobic cavities of protein,so as to use Xe as a heavy atom for determining phases in protein crystallography. Using single anomalous scattering diffraction method, we demonstrate