Single-Molecule STED Microscopy with Photostable Organic Fluorophores
Single-Molecule STED Microscopy with Photostable Organic Fluorophores
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DOI：
10.1002/smll.201000203
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发表时间：
2010-07-05
期刊：
影响因子：
13.3
通讯作者：
Sauer, Markus
中科院分区：
文献类型：
--
作者：
Kasper, Robert;Harke, Benjamin;Sauer, Markus
封面图像显示单个固定的双链寡核苷酸的荧光图像。荧光团的物理化学性质是突破衍射分辨率障碍的关键，因此，远场荧光纳米显微镜的发展关键取决于改进的荧光探针。受激发射耗尽（STED）显微镜相结合，通过还原和氧化系统（ROXS）的荧光团稳定，并证明了光稳定性的显着改善。这种改进可以用于3D或动态STED成像所需的重复测量，或者用于最大化其分辨率。在从固定在水性缓冲液中的单个有机荧光团记录的原始图像数据中证明了横向分辨率< 30 nm。欲了解更多信息，请阅读通讯“单分子STED显微镜与光稳定的有机荧光团”由P. Tinnefeld，SW地狱，M。Sauer等人，从1379页开始
The cover image shows fluorescence images of single immobilized double-stranded oligonucleotides. With photophysical and-chemical properties of fluorophores being the key to breaking the diffraction resolution barrier, the development of far-field fluorescence nanoscopy depends crucially on improved fluorescent probes. Stimulated emission depletion (STED) microscopy is combined with fluorophore stabilization through a reducing and oxidizing system (ROXS), and a significant improvement of photostability is demonstrated. This improvement can be exploited either for repetitive measurements necessary for 3D or dynamic STED imaging, or for maximizing its resolution. A lateral resolution< 30 nm is demonstrated in the raw image data recorded from single organic fluorophores immobilized in an aqueous buffer. For more information, please read the Communication “Single-Molecule STED Microscopy with Photostable Organic Fluorophores” by P. Tinnefeld, SW Hell, M. Sauer, et al., beginning on page 1379.