Timing and kinetics of E- to N-cadherin switch during neurulation in the avian embryo
Timing and kinetics of E- to N-cadherin switch during neurulation in the avian embryo
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DOI：
10.1002/dvdy.23813
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发表时间：
2012-08-01
影响因子：
2.5
通讯作者：
Duband, Jean-Loup
中科院分区：
文献类型：
--
作者：
Dady, Alwyn;Blavet, Cedrine;Duband, Jean-Loup
背景：在胚胎发育过程中，钙粘蛋白开关与组织重塑相关，如上皮-间充质转化（EMT）。在神经发生过程中也会发生E-到N-钙粘蛋白的转换，但这并不伴随EMT。这种开关的生物学意义目前尚不清楚。结果如下：我们分析了时间和动力学的E-N-钙粘蛋白开关在早期神经诱导和神经形成的鸡胚，在其转录调控模式。我们发现，部署的E-到N-钙粘蛋白开关程序有很大的变化沿着胚胎轴。在初级神经胚形成区域的喙部，它在时间和空间上以一种既不与神经上皮细胞的形态学转化有关也不与神经胚形成的运动同步的方式进行。尾部，在继发性神经元形成的区域，神经发生与钙粘蛋白开关不相关，因为N-钙粘蛋白在神经管形成之前就存在。我们还发现，在神经发育过程中，钙粘蛋白开关是由一组转录调节器精心策划的，这些转录调节器与参与EMT的转录调节器不同。结论：我们的研究结果表明，钙粘蛋白开关相关的分区的神经外胚层成其三个主要群体：外胚层，神经嵴，和神经管。发展动力学241：13331349，2012年。(c)2012 Wiley Periodicals，Inc.
Background: During embryonic development, cadherin switches are correlated with tissue remodelings, such as epithelium-to-mesenchyme transition (EMT). An E- to N-cadherin switch also occurs during neurogenesis, but this is not accompanied with EMT. The biological significance of this switch is currently unknown. Results: We analyzed the timing and kinetics of the E- to N-cadherin switch during early neural induction and neurulation in the chick embryo, in relation to the patterns of their transcriptional regulators. We found that deployment of the E- to N-cadherin switch program varies considerably along the embryonic axis. Rostrally in regions of primary neurulation, it occurs progressively both in time and space in a manner that appears neither in connection with morphological transformation of neural epithelial cells nor in synchrony with movements of neurulation. Caudally, in regions of secondary neurulation, neurogenesis was not associated with cadherin switch as N-cadherin pre-existed before formation of the neural tube. We also found that, during neural development, cadherin switch is orchestrated by a set of transcriptional regulators distinct from those involved in EMT. Conclusions: Our results indicate that cadherin switch correlates with the partition of the neurectoderm into its three main populations: ectoderm, neural crest, and neural tube. Developmental Dynamics 241:13331349, 2012. (c) 2012 Wiley Periodicals, Inc.