ANA screening: an old test with new recommendations
ANA screening: an old test with new recommendations
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DOI：
10.1136/ard.2009.127100
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发表时间：
2010-08-01
影响因子：
27.4
通讯作者：
Schur, Peter H.
中科院分区：
文献类型：
--
作者：
Meroni, Pier Luigi;Schur, Peter H.
从临床和实验室的角度来看，自身免疫性疾病的影响越来越大。诊断分析现在正从专门的专业实验室转移到高通量服务实验室。越来越多的可用方法增加了实验室之间的可变性，使它们的可重复性成为一个关键问题。另一方面，早期诊断和及时治疗需要可靠的检测，应减少重复确认性检测的费用，避免不必要的进一步调查。新的方法，特别是抗核抗体（ANAs），已经应用于自身抗体检测，以便比传统方法更快、更低成本地筛选和处理更大量的临床标本。然而，由于缺乏检测所有ANAs和标准化的敏感性，结果中存在不准确（包括假阳性和假阴性）的报告。美国风湿病学会成立了一个委员会来解决这个问题。已提出的具体建议代表了自身免疫性疾病诊断测试标准化的现实的第一步。
The impact of autoimmune diseases is growing from both a clinical and a laboratory point of view. Diagnostic assays are now being transferred from dedicated specialised laboratories into high-throughput service laboratories. The increasing number of available methods has raised the variability among the laboratories, making their reproducibility a critical problem. On the other hand, reliable tests are needed for early diagnosis and prompt treatment, and the cost of repeated confirmatory tests should be reduced and unnecessary further investigations avoided. New methodologies, particularly for antinuclear antibodies (ANAs), have been applied to autoantibody detection in order to screen and process larger volumes of clinical specimens more quickly and at less cost than the traditional methods. However, because of the lack of their sensitivity to detect all ANAs and standardisation, inaccuracies (including false positives and negatives) in the results have been reported. A committee of the American College of Rheumatology was formed to address this issue. Specific recommendations have been suggested that represent a realistic first step in the standardisation of diagnostic tests for autoimmune diseases.