Exploring and exploiting the synergy of non-covalent interactions on the surface of gold nanoparticles for fluorescent turn-on sensing of bacterial lipopolysaccharide
Exploring and exploiting the synergy of non-covalent interactions on the surface of gold nanoparticles for fluorescent turn-on sensing of bacterial lipopolysaccharide
复制标题
探索和利用金纳米粒子表面非共价相互作用的协同作用用于细菌脂多糖的荧光开启传感
DOI：
10.1039/c3nr02490c
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发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Zhao, Yibing
中科院分区：
文献类型：
--
作者：
Gao, Jinhong;Lai, Yangwei;Zhao, Yibing
脂多糖的传感依赖于脂多糖与传感器之间的多种静电和疏水相互作用的协同作用。然而，如何协调非共价相互作用来推动识别过程仍然是未知的，探索其中的哪一种将促进具有更高特异性和灵敏度的内毒素传感器的发展。在这项工作中，我们假设Au NPs将为研究非共价相互作用的协同作用提供一个直接而灵活的平台。系统地探索了所设计的荧光探针与具有两种不同表面性质的金纳米颗粒相互作用的详细机理。我们发现，只有当静电吸引和疏水堆积同时存在时，荧光探针才能与金纳米粒子高效结合，并具有正向协同作用。之后，利用由金纳米颗粒和表面组装的荧光探针组成的杂交系统来检测脂多糖的荧光开启。结果表明，荧光探针与金纳米颗粒的结合亲和力强烈依赖于荧光探针对内毒素的敏感性和选择性。因此，组装的荧光探针Au纳米颗粒为进一步优化LPS传感的灵敏度/选择性提供了一个有吸引力的平台。
The sensing of lipopolysaccharide (LPS) relies on the synergy of multiple electrostatic and hydrophobic interactions between LPS and the sensor. However, how non-covalent interactions are coordinated to impel the recognition process still remains elusive, and the exploration of which would promote the development of LPS sensors with higher specificity and sensitivity. In this work, we hypothesize that Au NPs would provide a straightforward and flexible platform for studying the synergy of non-covalent interactions. The detailed mechanism of interactions between the designed fluorescent probes and Au NPs with two distinct surface properties was systematically explored. We demonstrated that only when the electrostatic attraction and hydrophobic stacking are both present, the binding of fluorescent probes onto Au NPs can be not only highly efficient, but also positively cooperative. After that, hybrid systems that consist of Au NPs and surface-assembled fluorescent probes were exploited for fluorescent turn-on sensing of LPS. The results show that the sensitivity and selectivity to LPS relies strongly on the binding affinity between fluorescent probes and Au NPs. Fluorescent probes assembled Au NPs thus provide an attractive platform for further optimization of the sensitivity/selectivity of LPS sensing.