Salmonella Typhimurium is Attracted to Egg Yolk and Repelled by Albumen
Salmonella Typhimurium is Attracted to Egg Yolk and Repelled by Albumen
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DOI：
10.1007/s00284-018-1619-5
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发表时间：
2019-04-01
影响因子：
2.6
通讯作者：
Nakamura, Shuichi
中科院分区：
文献类型：
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作者：
Okuno, Katsuya;Xu, Jun;Nakamura, Shuichi
鼠伤寒沙门氏菌是非伤寒性食源性沙门氏菌病的病原体。鸡蛋被这种细菌污染是沙门氏菌的常见来源。鼠伤寒感染。S.鼠伤寒是围毛的，鞭毛依赖的运动性和趋化性被认为是促进鸡蛋污染，尽管存在许多抗菌鸡蛋成分。我们进行了运动和趋化性测定，以证明S。鼠伤寒沙门氏菌细胞被蛋黄吸引，被蛋白排斥。细菌鞭毛马达显示双向旋转，逆时针偏向旋转使细胞能够顺利游动。一个单一的鞭毛的旋转试验表明，与薄蛋白相比，厚蛋白更强烈地影响鞭毛旋转的方向偏差，导致在一个显着的抑制迁移距离。然而，S.鼠伤寒沙门氏菌细胞在蛋白存在下仍保持对卵黄的正趋化性，表明运动促进了沙门氏菌的生长。鼠伤寒和鸡蛋中的生存。
Salmonella Typhimurium is the causative agent of non-typhoidal, foodborne salmonellosis. Contamination of hen eggs by the bacterium is a common source of S. Typhimurium infection. S. Typhimurium is peritrichous, and flagellum-dependent motility and chemotaxis are believed to facilitate egg contamination despite the presence of many antimicrobial egg components. We performed motility and chemotaxis assays to demonstrate that S. Typhimurium cells are attracted to egg yolks and are repelled by albumen. The bacterial flagellar motor shows bidirectional rotation, and counterclockwise-biased rotation allows cells to swim smoothly. A rotation assay for a single flagellum showed that, in comparison with thin albumen, the thick albumen more strongly affected the directional bias of the flagellar rotation, resulting in a remarkable suppression of the migration distance. Nevertheless, the S. Typhimurium cells retained positive chemotaxis toward the yolk in the presence of the albumens, suggesting that motility facilitates the growth of S. Typhimurium and survival in eggs.