Electron detachment dissociation of fluorescently labeled sialylated oligosaccharides.
Electron detachment dissociation of fluorescently labeled sialylated oligosaccharides.
复制标题
荧光标记的唾液酸化寡糖的电子脱离解离。
DOI：
10.1002/elps.201100327
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
Hakansson,Kristina
中科院分区：
文献类型：
--
作者：
Zhou,Wen;Hakansson,Kristina
我们探索了电子分离解离（EDD）和红外多光子解离（IRMPD）串联质谱在荧光标记唾液酸寡糖中的应用。研究了从人转铁蛋白释放的标准唾液酸化低聚糖和唾液酸化N-连接聚糖。EDD产生了广泛的糖苷裂解和交叉环裂解在所有研究的情况下，始终提供互补的结构信息相比，红外多光子解离。EDD后还观察到中性离子损失和卫星离子，如C− 2 H离子。此外，我们研究了不同荧光标记的影响。酸性标记2-氨基苯甲酸（2-AA）在负离子模式下增强信号丰度。然而，对于2-AA-标记的寡糖，观察到很少的交叉环片段。中性标记2-氨基苯甲酰胺（2-AB）导致比2-AA-标记物质更多的交叉环裂解，但不像天然寡糖那样广泛的片段化，可能是由于引入荧光标签改变了负电荷位置。
We explored the application of electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry to fluorescently labeled sialylated oligosaccharides. Standard sialylated oligosaccharides and a sialylated N ‐linked glycan released from human transferrin were investigated. EDD yielded extensive glycosidic cleavages and cross‐ring cleavages in all cases studied, consistently providing complementary structural information compared with infrared multiphoton dissociation. Neutral losses and satellite ions such as C−2H ions were also observed following EDD. In addition, we examined the influence of different fluorescent labels. The acidic label 2‐aminobenzoic acid (2‐AA) enhanced signal abundance in negative‐ion mode. However, few cross‐ring fragments were observed for 2‐AA‐labeled oligosaccharides. The neutral label 2‐aminobenzamide (2‐AB) resulted in more cross‐ring cleavages compared with 2‐AA‐labeled species, but not as extensive fragmentation as for native oligosaccharides, likely resulting from altered negative charge locations from introduction of the fluorescent tag.