Identification of lipases involved in PBAN stimulated pheromone production in Bombyx mori using the DGE and RNAi approaches.
Identification of lipases involved in PBAN stimulated pheromone production in Bombyx mori using the DGE and RNAi approaches.
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DOI：
10.1371/journal.pone.0031045
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
An S
中科院分区：
文献类型：
--
作者：
Du M;Yin X;Zhang S;Zhu B;Song Q;An S
信息素生物合成激活神经肽（Pheromone biosynthesis activating neuropeptide，PBAN）是一种调节雌蛾性信息素合成的神经激素。家蚕是一种模式生物，已被用来探索PBAN的信号转导模式，这是由G蛋白偶联受体（GPCR）介导的。虽然在阐明PBAN调节的脂解释放性信息素的前体方面取得了重大进展，但对这一步骤中涉及的分子组分知之甚少。为了更好地阐明PBAN刺激的细胞质脂滴（LD）的脂解的分子机制，使用数字基因表达（DGE）和随后的RNA干扰（RNAi）鉴定了参与PBAN调节的性信息素生物合成的相关脂肪酶基因。以羽化前72 h（-72 h）、羽化后72 h（0 h）和羽化后72 h（72 h）的信息素腺体为材料，构建3个DGE文库，研究信息素腺体发育过程中的基因表达谱。DGE评估了每个PG样本中超过560万个干净标签，并揭示了在这些阶段差异表达的许多基因。最重要的是，发现7种脂肪酶在性信息素合成和释放的关键阶段（新出现）丰富表达。RNAi介导的敲除首次证实了这七种脂肪酶中的四种在性信息素合成中起重要作用。这项研究已经确定了四个脂肪酶直接参与PBAN刺激的性信息素的生物合成，提高我们的理解，参与释放三酰甘油（TAG）的细胞质LD内的家蚕酚前体的脂肪酶。
Pheromone biosynthesis activating neuropeptide (PBAN) is a neurohormone that regulates sex pheromone synthesis in female moths. Bombyx mori is a model organism that has been used to explore the signal transduction pattern of PBAN, which is mediated by a G-protein coupled receptor (GPCR). Although significant progress has been made in elucidating PBAN-regulated lipolysis that releases the precursor of the sex pheromone, little is known about the molecular components involved in this step. To better elucidate the molecular mechanisms of PBAN-stimulated lipolysis of cytoplasmic lipid droplets (LDs), the associated lipase genes involved in PBAN- regulated sex pheromone biosynthesis were identified using digital gene expression (DGE) and subsequent RNA interference (RNAi). Three DGE libraries were constructed from pheromone glands (PGs) at different developed stages, namely, 72 hours before eclosion (−72 h), new emergence (0 h) and 72 h after eclosion (72 h), to investigate the gene expression profiles during PG development. The DGE evaluated over 5.6 million clean tags in each PG sample and revealed numerous genes that were differentially expressed at these stages. Most importantly, seven lipases were found to be richly expressed during the key stage of sex pheromone synthesis and release (new emergence). RNAi-mediated knockdown confirmed for the first time that four of these seven lipases play important roles in sex pheromone synthesis. This study has identified four lipases directly involved in PBAN-stimulated sex pheromone biosynthesis, which improve our understanding of the lipases involved in releasing bombykol precursors from triacylglycerols (TAGs) within the cytoplasmic LDs.
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影响因子：
7
作者：
Morrissy, A. Sorana;Morin, Ryan D.;Marra, Marco A.
通讯作者：
Marra, Marco A.
影响因子：
4.8
作者：
Haemmerle, G;Zimmermann, R;Zechner, R
通讯作者：
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影响因子：
4.8
作者：
Ohnishi, Atsushi;Hull, J. Joe;Matsumoto, Shogo
通讯作者：
Matsumoto, Shogo
影响因子：
4.8
作者：
Ohnishi, Atsushi;Hashimoto, Kana;Matsumoto, Shogo
通讯作者：
Matsumoto, Shogo
DOI：
10.1073/pnas.97.2.787
发表时间：
2000-01-18
影响因子：
11.1
作者：
Osuga, J;Ishibashi, S;Yamada, N
通讯作者：
Yamada, N